Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,362	C→T	A84V (GCC→GTC)	mltG →	endolytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,362	0	C	T	100.0%	116.6 / NA	29	A84V (GCC→GTC)	mltG	endolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/15);  total (14/15)

TCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCT  >  NC_000913/1154318‑1154407                                             |                                              tCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCgg                                            >  1:629717/1‑48 (MQ=255)  cAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCggg                                           <  1:1202054/48‑1 (MQ=255)     tGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACtt                                       <  1:2756545/49‑1 (MQ=255)      ggCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTa                                      >  1:241708/1‑49 (MQ=255)       gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTa                                      <  1:348310/48‑1 (MQ=255)       gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAc                                     <  1:2545960/49‑1 (MQ=255)        ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                    <  1:518875/49‑1 (MQ=255)        ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                    <  1:2373213/49‑1 (MQ=255)          gctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGc                                  >  1:164838/1‑49 (MQ=255)           ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCg                                   <  1:246124/47‑1 (MQ=255)              cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCttt                               <  1:1212609/48‑1 (MQ=255)               gTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTa                              >  1:1045045/1‑48 (MQ=255)                 aTCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTacac                           >  1:393581/1‑49 (MQ=255)                  tCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACAcc                          >  1:1313705/1‑49 (MQ=255)                      aCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAg                      <  1:974413/49‑1 (MQ=255)                      aCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAg                      <  1:2606145/49‑1 (MQ=255)                        cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                    >  1:2174302/1‑49 (MQ=255)                         ggATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                    >  1:1415929/1‑48 (MQ=255)                          gATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGa                  >  1:1567538/1‑49 (MQ=255)                            tCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc                >  1:1950815/1‑49 (MQ=255)                                tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc                <  1:1306831/45‑1 (MQ=255)                                 ctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcg           >  1:2807884/1‑49 (MQ=255)                                 ctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcg           >  1:1488140/1‑49 (MQ=255)                                    aCTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcg           <  1:1825524/46‑1 (MQ=255)                                       ttAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGAt     >  1:1777096/1‑49 (MQ=255)                                        tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGAt     <  1:1434805/48‑1 (MQ=255)                                          aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCt  <  1:2385906/49‑1 (MQ=255)                                          aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCt  >  1:1744485/1‑49 (MQ=255)                                          aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCt  <  1:396944/49‑1 (MQ=255)                                             |                                              TCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCT  >  NC_000913/1154318‑1154407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑