Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,240,439	C→T	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM → cvrA ←	protein YmgM putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,240,439	0	C	T	100.0%	72.8 / NA	19	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM cvrA	protein YmgM putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/13);  total (6/13)

TTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTC  >  NC_000913/1240396‑1240481                                            |                                           ttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGa                                       >  1:385055/1‑49 (MQ=255)   ctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGAc                                      >  1:1416153/1‑48 (MQ=255)     cGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAAc                                   >  1:1219413/1‑49 (MQ=255)      ggCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAAc                                   <  1:2765279/48‑1 (MQ=255)       gCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCg                                 <  1:1186086/49‑1 (MQ=255)       gCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCg                                 <  1:2636351/49‑1 (MQ=255)         cACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGt                               <  1:485163/49‑1 (MQ=255)             gTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCcg                           <  1:1496592/49‑1 (MQ=255)               ccAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCcgcg                         >  1:2790531/1‑49 (MQ=255)                 aGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGcc                       <  1:2362121/49‑1 (MQ=255)                  gATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCt                      <  1:2736871/49‑1 (MQ=255)                   atcatcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCt                      >  1:1319895/1‑48 (MQ=255)                      atcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaaca                  <  1:452227/49‑1 (MQ=255)                       tcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaacaa                 <  1:293459/49‑1 (MQ=255)                          cGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActg              <  1:1526155/49‑1 (MQ=255)                           ggCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgc             >  1:1047688/1‑49 (MQ=255)                             cAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgctg           <  1:263603/49‑1 (MQ=255)                                 ctctACCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACa       <  1:408554/49‑1 (MQ=255)                                      ccTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTc  <  1:275427/49‑1 (MQ=255)                                            |                                           TTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTC  >  NC_000913/1240396‑1240481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑