Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	122.5 / NA	31	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270765‑1270856                                               |                                              atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             <  1:2444962/49‑1 (MQ=255)  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:2790052/1‑48 (MQ=255)   caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:1528763/1‑47 (MQ=255)    aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          <  1:1495400/49‑1 (MQ=255)     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                         <  1:2516952/49‑1 (MQ=255)        aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                      >  1:391480/1‑49 (MQ=255)          gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                    <  1:1154836/49‑1 (MQ=255)             gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 <  1:2215628/49‑1 (MQ=255)             gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 >  1:1540918/1‑49 (MQ=255)              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                >  1:523284/1‑49 (MQ=255)              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                <  1:1657576/49‑1 (MQ=255)                aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                              <  1:2521286/49‑1 (MQ=255)                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:1005438/1‑47 (MQ=255)                 aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:1780994/1‑47 (MQ=255)                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:2428177/1‑46 (MQ=255)                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                     >  1:2761484/1‑48 (MQ=255)                          gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                    <  1:1928184/49‑1 (MQ=255)                           ctgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   >  1:2213906/2‑49 (MQ=255)                           aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   >  1:707930/1‑49 (MQ=255)                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                <  1:173254/49‑1 (MQ=255)                              cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                 <  1:2093857/48‑1 (MQ=255)                                ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:1857196/1‑49 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca            <  1:1002980/48‑1 (MQ=255)                                     ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa         <  1:2391333/49‑1 (MQ=255)                                      cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:1960262/1‑49 (MQ=255)                                         tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:377448/1‑48 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa     <  1:1304482/48‑1 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:630382/1‑47 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:886470/1‑47 (MQ=255)                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa    >  1:1261167/1‑47 (MQ=255)                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga  <  1:2575246/49‑1 (MQ=255)                                               |                                              ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270765‑1270856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑