Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	114.8 / NA	30	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/14);  total (16/14)

AGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTA  >  NC_000913/118984‑119069                                             |                                          aGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                       >  1:2759061/1‑49 (MQ=255)       gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                 >  1:811451/1‑49 (MQ=255)          tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                              <  1:1616086/49‑1 (MQ=255)           gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                             >  1:2840509/1‑49 (MQ=255)             tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                           >  1:484592/1‑49 (MQ=255)              ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                           <  1:599679/48‑1 (MQ=255)              ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                          >  1:405264/1‑49 (MQ=255)               cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                         >  1:643306/1‑49 (MQ=255)               cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                          <  1:846034/48‑1 (MQ=255)               cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                          <  1:2015082/48‑1 (MQ=255)                tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                        >  1:2713386/1‑49 (MQ=255)                 ttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                        >  1:2088682/1‑48 (MQ=255)                   cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa                     >  1:2222019/1‑49 (MQ=255)                   cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa                     >  1:920518/1‑49 (MQ=255)                    gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                    <  1:1851579/49‑1 (MQ=255)                     aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                   <  1:1791490/49‑1 (MQ=255)                      tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                  >  1:197945/1‑49 (MQ=255)                      tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                  >  1:528702/1‑49 (MQ=255)                      tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                  >  1:1641443/1‑49 (MQ=255)                        aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                 <  1:248794/48‑1 (MQ=255)                         aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc               <  1:147515/49‑1 (MQ=255)                          cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCa              <  1:1482783/49‑1 (MQ=255)                              catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa          <  1:315964/49‑1 (MQ=255)                              catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa          <  1:2834885/49‑1 (MQ=255)                               atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt         >  1:2717087/1‑49 (MQ=255)                               atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAt         >  1:222238/1‑49 (MQ=255)                                 ggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt       >  1:640901/1‑49 (MQ=255)                                   catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt     >  1:379438/1‑49 (MQ=255)                                     tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt   <  1:35027/49‑1 (MQ=255)                                     tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt   <  1:1290917/49‑1 (MQ=255)                                       cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTa  <  1:1710205/48‑1 (MQ=255)                                             |                                          AGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTA  >  NC_000913/118984‑119069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑