Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,662,872	C→T	R773W (CGG→TGG)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,662,872	0	C	T	100.0%	97.9 / NA	25	R773W (CGG→TGG)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

CGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCA  >  NC_000913/1662826‑1662912                                               |                                         cGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTgg                                        <  1:949888/49‑1 (MQ=255)  gCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTggg                                       <  1:1826648/49‑1 (MQ=255)   ccATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGt                                      <  1:196179/49‑1 (MQ=255)    cATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTc                                     <  1:2312736/49‑1 (MQ=255)     aTCGGGCAATGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTc                                     >  1:1497672/1‑48 (MQ=38)      tCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGa                                   <  1:2654433/49‑1 (MQ=255)        gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                  >  1:2546519/1‑48 (MQ=255)        gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                  >  1:2349047/1‑48 (MQ=255)        gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                  >  1:2437179/1‑48 (MQ=255)        gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATc                                 <  1:450659/49‑1 (MQ=255)        gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATc                                 <  1:2011318/49‑1 (MQ=255)          gCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAc                               <  1:2358191/49‑1 (MQ=255)            aaGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcgg                             >  1:1290846/1‑49 (MQ=255)              ggTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggcg                           >  1:2024060/1‑49 (MQ=255)                tGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACggcggc                         <  1:1221899/49‑1 (MQ=255)                   gTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGt                      <  1:656315/49‑1 (MQ=255)                   gTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGt                      <  1:1111725/49‑1 (MQ=255)                    tGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTa                     >  1:949942/1‑49 (MQ=255)                     ggCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTAt                    >  1:2342590/1‑49 (MQ=255)                          aaaGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAat                <  1:397713/48‑1 (MQ=255)                           aaGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAatat              >  1:326895/1‑49 (MQ=255)                           aaGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAata               <  1:2175476/48‑1 (MQ=255)                              cGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCt           <  1:2549580/49‑1 (MQ=255)                                   atGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACct      >  1:1778844/1‑49 (MQ=255)                                       ttGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCa  >  1:547871/1‑49 (MQ=255)                                               |                                         CGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCA  >  NC_000913/1662826‑1662912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑