Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,697,216	T→C	intergenic (‑164/+57)	uidR ← / ← hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,697,216	0	T	C	100.0%	146.7 / NA	37	intergenic (‑164/+57)	uidR/hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/22);  total (15/22)

GTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGG  >  NC_000913/1697170‑1697260                                               |                                             gTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtg                                            <  1:2813975/49‑1 (MQ=255)       cAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGt                                      <  1:903973/49‑1 (MQ=255)       cAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGt                                      >  1:2337324/1‑49 (MQ=255)         aTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGtt                                     >  1:774581/1‑48 (MQ=255)          tAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTa                                   <  1:215690/49‑1 (MQ=255)            gCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTAt                                  <  1:270061/48‑1 (MQ=255)            gCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATc                                 >  1:256733/1‑49 (MQ=255)            gCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATc                                 <  1:2468219/49‑1 (MQ=255)            gCCGTGATTTGGCTGCCAGTTATTTAGCAGAGAAGCTGATGAGTTTATc                                 >  1:2647210/1‑49 (MQ=255)              cGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATccc                               <  1:799847/49‑1 (MQ=255)               gTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                              >  1:1151776/1‑49 (MQ=255)                 gATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCgg                             >  1:2097071/1‑48 (MQ=255)                  aTTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTa                           <  1:53693/49‑1 (MQ=255)                  aTTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTa                           <  1:363737/49‑1 (MQ=255)                   tttGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaa                          <  1:2691945/49‑1 (MQ=255)                    ttGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaaa                         >  1:2147398/1‑49 (MQ=255)                    ttGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaaa                         >  1:552824/1‑49 (MQ=255)                      ggCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaac                        >  1:140517/1‑48 (MQ=255)                         tGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAacaaca                    >  1:2131514/1‑49 (MQ=255)                           ccAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacact                  <  1:2014132/49‑2 (MQ=255)                           ccAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacg                  <  1:299765/49‑1 (MQ=255)                            cAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacg                  >  1:906270/1‑48 (MQ=255)                              gTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac               >  1:1790037/1‑49 (MQ=255)                              gTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac               <  1:1714355/49‑1 (MQ=255)                              gTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac               <  1:2816541/49‑1 (MQ=255)                               ttATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcacg              <  1:1110292/49‑1 (MQ=255)                               ttATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcacg              <  1:2128316/49‑1 (MQ=255)                                tATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACgg             >  1:1041240/1‑49 (MQ=255)                                  tttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgtt           >  1:1960315/1‑49 (MQ=255)                                     aGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgtt        >  1:988328/1‑49 (MQ=255)                                       cAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTa      <  1:2650679/49‑1 (MQ=255)                                         gagaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc    <  1:2073429/49‑1 (MQ=255)                                         gagaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc    <  1:1943788/49‑1 (MQ=255)                                         gagaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc    <  1:1479182/49‑1 (MQ=255)                                         gagaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc    <  1:1282794/49‑1 (MQ=255)                                          agaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCg   <  1:903533/49‑1 (MQ=255)                                           gaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCgg  <  1:439421/49‑1 (MQ=255)                                               |                                             GTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGG  >  NC_000913/1697170‑1697260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑