Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,741,062	T→C	C384C (TGT→TGC)	ydhC →	putative transporter YdhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,741,062	0	T	C	100.0%	91.2 / NA	24	C384C (TGT→TGC)	ydhC	putative transporter YdhC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/6);  total (18/6)

TAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCAT  >  NC_000913/1741025‑1741107                                      |                                              tagtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTgg                                    >  1:229111/1‑49 (MQ=255) tagtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTgg                                    >  1:1939916/1‑49 (MQ=255)  agtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGc                                   >  1:479252/1‑49 (MQ=255)         gTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAg                             >  1:2142216/1‑48 (MQ=255)              gcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCAt                       >  1:813456/1‑49 (MQ=255)              gcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCAt                       >  1:2751155/1‑49 (MQ=255)              gcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCAt                       >  1:2829820/1‑49 (MQ=255)                tGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATgg                     >  1:1082873/1‑49 (MQ=255)                 gggTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATgg                     >  1:42923/1‑48 (MQ=255)                    ttACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAAt                 <  1:2466242/49‑1 (MQ=255)                      aCATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGc               >  1:2380573/1‑49 (MQ=255)                       cATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGcc              >  1:2324375/1‑49 (MQ=255)                        atgatgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGcc              >  1:1289158/1‑48 (MQ=255)                        atgatgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCg             <  1:1945994/49‑1 (MQ=255)                           atgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAg          <  1:1898955/49‑1 (MQ=255)                           atgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAg          <  1:2691839/49‑1 (MQ=255)                            tgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGt         >  1:240682/1‑49 (MQ=255)                            tgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGt         >  1:1472272/1‑49 (MQ=255)                             gCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTc        >  1:2575449/1‑49 (MQ=255)                               aaCGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGc      >  1:2451978/1‑49 (MQ=255)                               aaCGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGc      <  1:288246/49‑1 (MQ=255)                               aaCGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGc      >  1:1085962/1‑49 (MQ=255)                                  gTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCa   >  1:55513/1‑49 (MQ=255)                                   ttGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCAt  <  1:1126982/49‑1 (MQ=255)                                      |                                              TAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCAT  >  NC_000913/1741025‑1741107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑