Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	119.6 / NA	31	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/16);  total (15/16)

CGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACG  >  NC_000913/1742711‑1742799                                             |                                             cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                          <  1:1913512/49‑1 (MQ=255)   ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                         >  1:2824014/1‑48 (MQ=255)    cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                                       <  1:1913176/49‑1 (MQ=255)       gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                    >  1:703049/1‑49 (MQ=255)        ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATgg                                   <  1:1773479/49‑1 (MQ=255)        ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                    >  1:808427/1‑48 (MQ=255)          ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                  <  1:1548064/48‑1 (MQ=255)             tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                               <  1:367003/48‑1 (MQ=255)              cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                              <  1:600356/48‑1 (MQ=255)              cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                              >  1:457580/1‑48 (MQ=255)                 cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                            <  1:891778/47‑1 (MQ=255)                 cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                             <  1:738754/46‑1 (MQ=255)                   cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                        <  1:1712376/49‑1 (MQ=255)                    aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                       >  1:2476884/1‑49 (MQ=255)                     agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                      >  1:1309720/1‑49 (MQ=255)                      gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                     >  1:1911184/1‑49 (MQ=255)                       agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                    <  1:361174/49‑1 (MQ=255)                       agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                    <  1:1272288/49‑1 (MQ=255)                         tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                  <  1:1715609/49‑1 (MQ=255)                           gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                <  1:1947583/49‑1 (MQ=255)                            tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt               >  1:2162262/1‑49 (MQ=255)                               gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc            >  1:2120289/1‑49 (MQ=255)                               gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc            <  1:1700208/49‑1 (MQ=255)                                   cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc         <  1:1938513/48‑1 (MQ=255)                                   cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc         <  1:887258/48‑1 (MQ=255)                                    aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc       >  1:2030217/1‑49 (MQ=255)                                      tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc      >  1:54925/1‑48 (MQ=255)                                       gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa    >  1:202139/1‑49 (MQ=255)                                        tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc   >  1:2759988/1‑49 (MQ=255)                                         ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg  >  1:2441762/1‑49 (MQ=255)                                         ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg  >  1:1573811/1‑49 (MQ=255)                                             |                                             CGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACG  >  NC_000913/1742711‑1742799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑