Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,533	G→A	G316D (GGC→GAC)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,533	0	G	A	100.0%	84.4 / NA	22	G316D (GGC→GAC)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/9);  total (13/9)

AGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAC  >  NC_000913/1776489‑1776578                                             |                                              aGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTg                                           <  1:1313633/49‑1 (MQ=255) aGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTg                                           <  1:506416/49‑1 (MQ=255)       ggTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGcc                                     >  1:1547924/1‑49 (MQ=255)       ggTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGcc                                     <  1:2528730/49‑1 (MQ=255)         tGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                    >  1:463844/1‑48 (MQ=255)              aaTGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGagaag                              <  1:739816/49‑1 (MQ=255)                tGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGagaag                              >  1:2614121/1‑47 (MQ=255)                tGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAgaagaa                            <  1:1750412/49‑1 (MQ=255)                  tCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAgg                          >  1:1621280/1‑49 (MQ=255)                  tCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAgg                          <  1:2808941/49‑1 (MQ=255)                      cGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgt                      >  1:617439/1‑49 (MQ=255)                       gTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgtg                     >  1:481803/1‑49 (MQ=255)                       gTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgtg                     <  1:2827751/49‑1 (MQ=255)                       gTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgtg                     >  1:1035743/1‑49 (MQ=255)                          ggTATTGCTGACGGCATTGACCTGGTCGCCCGAG‑‑GAAGGTTGTGCtgat                >  1:1608949/1‑49 (MQ=37)                            tATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgat                >  1:1381132/1‑49 (MQ=255)                               tGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGAc             >  1:980210/1‑49 (MQ=255)                                 cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt           >  1:2479357/1‑49 (MQ=255)                                 cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACt            <  1:565558/48‑1 (MQ=255)                                    aCGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTAt        >  1:305095/1‑49 (MQ=255)                                       gCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCt     >  1:1197122/1‑49 (MQ=255)                                          ttGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAc  <  1:1540602/49‑1 (MQ=255)                                             |                                              AGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAC  >  NC_000913/1776489‑1776578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑