Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,898,152	G→A	A407A (GCG→GCA)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,898,152	0	G	A	100.0%	48.0 / NA	13	A407A (GCG→GCA)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

GCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTC  >  NC_000913/1898106‑1898190                                               |                                       gCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCcgcacg                                      >  1:1317828/1‑49 (MQ=255)  cATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCcgcacg                                      >  1:436903/1‑48 (MQ=255)       aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATgg                                 <  1:1832669/48‑1 (MQ=255)       aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGc                                >  1:520010/1‑49 (MQ=255)       aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGc                                >  1:870269/1‑49 (MQ=255)         cGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctc                              <  1:1512832/49‑1 (MQ=255)          gTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctct                             >  1:1140209/1‑49 (MQ=255)           tAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTg                            >  1:635805/1‑49 (MQ=255)                     gCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCAc                    >  1:2145864/1‑47 (MQ=255)                       ctctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGt                >  1:1402674/1‑49 (MQ=255)                          tgtgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCa             >  1:791236/1‑49 (MQ=255)                            tgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGc              <  1:2364237/46‑1 (MQ=255)                                    aTTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtct   >  1:2844761/1‑49 (MQ=255)                                     ttAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc  <  1:1930600/49‑1 (MQ=255)                                     ttAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc  <  1:2107325/49‑1 (MQ=255)                                               |                                       GCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTC  >  NC_000913/1898106‑1898190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑