Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,784	A→G	V267A (GTC→GCC)	znuA ←	Zn(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,784	0	A	G	100.0%	52.1 / NA	15	V267A (GTC→GCC)	znuA	Zn(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/3);  total (12/3)

CCCAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAA  >  NC_000913/1941739‑1941826                                              |                                           cccAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGgcgc                                         >  1:105425/1‑49 (MQ=255)  ccAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGct                                        >  1:1901619/1‑49 (MQ=255)        gATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAAc                                  >  1:1259004/1‑49 (MQ=255)         aTCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAAc                                  >  1:1973221/1‑48 (MQ=255)          tCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGa                                <  1:834248/49‑1 (MQ=255)               cGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCt                           >  1:687922/1‑49 (MQ=255)                gTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTg                          >  1:272160/1‑49 (MQ=255)                  tCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGc                        >  1:2158644/1‑49 (MQ=255)                   cccATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGcc                       >  1:1239889/1‑49 (MQ=255)                   cccATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGcc                       >  1:194973/1‑49 (MQ=255)                          gAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACtg                <  1:434148/49‑1 (MQ=255)                            aCGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACtgtg              <  1:1968529/49‑1 (MQ=255)                            aCGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACtgtg              >  1:836211/1‑49 (MQ=255)                             cGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTgg             >  1:2644870/1‑49 (MQ=255)                               gATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt           >  1:2854915/1‑49 (MQ=255)                                        cGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaaaa  >  1:2411272/1‑49 (MQ=255)                                              |                                           CCCAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAA  >  NC_000913/1941739‑1941826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑