Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,885	C→T	T18I (ACC→ATC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,885	0	C	T	100.0%	100.4 / NA	25	T18I (ACC→ATC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/17);  total (8/17)

GTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGA  >  NC_000913/214839‑214929                                               |                                             gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                            >  1:1142396/1‑49 (MQ=255) gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                            >  1:1843403/1‑49 (MQ=255)  tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                            <  1:144528/48‑1 (MQ=255)    aTTTCCCGACATGTTGCTATTTCCGATGGTGAGCTTGAGATCATCGCCa                                         >  1:464132/1‑49 (MQ=255)     tttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                        >  1:2851753/1‑49 (MQ=255)        cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                      <  1:2721566/48‑1 (MQ=255)        cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                     >  1:414179/1‑49 (MQ=255)        cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                     >  1:504160/1‑49 (MQ=255)          cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                   <  1:430388/49‑1 (MQ=255)          cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                   <  1:2832802/49‑1 (MQ=255)           gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                  <  1:2643736/49‑1 (MQ=255)               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                              <  1:1399572/49‑1 (MQ=255)                 ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                             <  1:1950674/48‑1 (MQ=255)                 ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                             <  1:256472/48‑1 (MQ=255)                  tGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                           >  1:1886092/1‑49 (MQ=255)                       ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                        <  1:2386481/47‑1 (MQ=255)                         cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                     <  1:1041050/48‑1 (MQ=255)                         cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                     <  1:1508227/48‑1 (MQ=255)                           cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                  >  1:488943/1‑49 (MQ=255)                                 tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCAt            <  1:864051/49‑1 (MQ=255)                                    gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt         <  1:1709060/49‑1 (MQ=255)                                     cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta        <  1:2678824/49‑1 (MQ=255)                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaataa    <  1:2421714/49‑1 (MQ=255)                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata     <  1:1614928/48‑1 (MQ=255)                                          gaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAg   <  1:1007162/49‑1 (MQ=255)                                           atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGa  >  1:912336/1‑49 (MQ=255)                                               |                                             GTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGA  >  NC_000913/214839‑214929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑