Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,004	T→C	E43G (GAA→GGA)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,004	0	T	C	100.0%	101.8 / NA	26	E43G (GAA→GGA)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/16);  total (10/16)

CGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATC  >  NC_000913/2661960‑2662044                                             |                                         cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCa                                       <  1:2738380/48‑1 (MQ=255) cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCa                                       <  1:1706729/48‑1 (MQ=255) cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCa                                       <  1:1786832/48‑1 (MQ=255)   cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                    <  1:2807549/49‑1 (MQ=255)    tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                    >  1:2364080/1‑48 (MQ=255)     ggAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                    >  1:1983817/1‑47 (MQ=255)       aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg                                 >  1:1068453/1‑48 (MQ=255)       aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg                                 >  1:1803423/1‑48 (MQ=255)       aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                <  1:666000/49‑1 (MQ=255)        aaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaa                               <  1:969493/49‑1 (MQ=255)        aaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaa                               <  1:1841411/49‑1 (MQ=255)            aGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAggg                           <  1:1377643/49‑1 (MQ=255)            aGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg                            >  1:469271/1‑48 (MQ=255)                cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAt                       <  1:2703662/49‑1 (MQ=255)                 aTTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt                      <  1:1722557/49‑1 (MQ=255)                 aTTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt                      >  1:1754039/1‑49 (MQ=255)                       aCGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTg                 <  1:2705523/48‑1 (MQ=255)                           aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt            <  1:2141315/49‑1 (MQ=255)                             aCGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg          <  1:1593468/49‑1 (MQ=255)                               gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg         >  1:2306219/1‑48 (MQ=255)                               gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg         >  1:1883907/1‑48 (MQ=255)                               gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa        <  1:1019606/49‑1 (MQ=255)                                ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaa       <  1:2689921/49‑1 (MQ=255)                                ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa        >  1:1394539/1‑48 (MQ=255)                                    aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat   >  1:122800/1‑49 (MQ=255)                                     aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc  <  1:240663/49‑1 (MQ=255)                                             |                                         CGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATC  >  NC_000913/2661960‑2662044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑