Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	113.9 / NA	30	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/14);  total (16/14)

GGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512958‑3513048                                              |                                              ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:1198822/1‑49 (MQ=255)    gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  1:1351388/49‑1 (MQ=255)    gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  1:1387566/49‑1 (MQ=255)        gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  1:2459957/49‑1 (MQ=255)         tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    <  1:596071/49‑1 (MQ=255)         tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    >  1:2438753/1‑49 (MQ=255)          gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  1:1322135/1‑49 (MQ=255)            ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:2643937/49‑1 (MQ=255)                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:2511818/1‑48 (MQ=255)                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:2866353/49‑1 (MQ=255)                  gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           <  1:1025270/49‑1 (MQ=255)                  gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           >  1:2467945/1‑49 (MQ=255)                     tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:2763195/1‑48 (MQ=255)                      gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGtt                        <  1:610472/48‑1 (MQ=255)                       aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      >  1:1336008/1‑49 (MQ=255)                           tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   <  1:2491837/48‑1 (MQ=255)                             ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:2238674/1‑49 (MQ=255)                              tcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:495311/1‑48 (MQ=255)                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAtt               <  1:2063151/48‑1 (MQ=255)                                aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:733712/1‑49 (MQ=255)                                aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:1715857/1‑49 (MQ=255)                                aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:2543421/1‑49 (MQ=255)                                 cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:2200009/49‑1 (MQ=255)                                  tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           >  1:1643397/1‑49 (MQ=255)                                    ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           <  1:2623355/47‑1 (MQ=255)                                     cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCa        <  1:1137548/49‑1 (MQ=255)                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:2821805/1‑46 (MQ=255)                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:1927260/1‑46 (MQ=255)                                           cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   <  1:278748/48‑1 (MQ=255)                                           cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:878212/1‑49 (MQ=255)                                              |                                              GGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512958‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑