Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,871,692	C→T	R13R (CGG→CGA)	dgoT ←	putative D‑galactonate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,871,692	0	C	T	100.0%	137.2 / NA	34	R13R (CGG→CGA)	dgoT	putative D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/17);  total (17/17)

ACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGA  >  NC_000913/3871647‑3871735                                              |                                            aCAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGa                                          >  1:1970939/1‑49 (MQ=255)  cAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGAc                                         <  1:2042401/49‑1 (MQ=255)    aaTGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGc                                       >  1:766383/1‑49 (MQ=255)     aTGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGcc                                      >  1:868903/1‑49 (MQ=255)        aCTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGcccc                                    <  1:1992635/48‑1 (MQ=255)        aCTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCg                                   <  1:65743/49‑1 (MQ=255)         cTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCgg                                  <  1:154301/49‑1 (MQ=255)         cTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCgg                                  >  1:649621/1‑49 (MQ=255)            ccGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGc                                 <  1:2490682/47‑1 (MQ=255)            ccGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCtt                               >  1:653589/1‑49 (MQ=255)             cGTAATAAAGATCATCACCAGCGTCGGATAACGTCGACGCCCCGGCttt                              <  1:1042622/49‑1 (MQ=39)               taataaAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTtgc                            >  1:442083/1‑49 (MQ=255)                  taaAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTtgctg                          >  1:1229430/1‑48 (MQ=255)                   aaaGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCa                        >  1:1956947/1‑49 (MQ=255)                    aaGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCAt                       >  1:174815/1‑49 (MQ=255)                     aGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCAtt                      <  1:2375096/49‑1 (MQ=255)                         catcaCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACg                  >  1:2398003/1‑49 (MQ=255)                          atcaCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACgg                 <  1:2662070/49‑1 (MQ=255)                          atcaCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACg                  <  1:2441306/48‑1 (MQ=255)                           tcaCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACggg                <  1:2104658/49‑1 (MQ=255)                            caCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACggg                >  1:1229612/1‑48 (MQ=255)                              ccAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGaa              >  1:410830/1‑48 (MQ=255)                              ccAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAat             <  1:639394/49‑1 (MQ=255)                               cAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAata            <  1:1655153/49‑1 (MQ=255)                                aGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAatat           >  1:1936957/1‑49 (MQ=255)                                   gTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCa        >  1:179018/1‑49 (MQ=255)                                   gTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCa        <  1:689448/49‑1 (MQ=255)                                   gTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCa        >  1:1466832/1‑49 (MQ=255)                                   gTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCa        >  1:2294313/1‑49 (MQ=255)                                    tCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCAt       <  1:490957/49‑1 (MQ=255)                                    tCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCAt       <  1:527270/49‑1 (MQ=255)                                      aGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAg     <  1:2563588/49‑1 (MQ=255)                                        aTAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGc    >  1:403912/1‑48 (MQ=255)                                         tAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGa  <  1:2373090/49‑1 (MQ=255)                                              |                                            ACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGA  >  NC_000913/3871647‑3871735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑