Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	98.8 / NA	22	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/12);  total (10/12)

TTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAA  >  NC_000913/765143‑765230                                               |                                          ttttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                         >  1:1587851/1‑48 (MQ=255)   ttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                         <  1:1355288/47‑2 (MQ=255)      tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                    <  1:1734826/48‑1 (MQ=38)        tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                 <  1:941174/49‑1 (MQ=38)        tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                 >  1:1936967/1‑49 (MQ=38)          cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                <  1:1435563/48‑1 (MQ=38)          cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                               <  1:1944503/49‑1 (MQ=38)              tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                            <  1:1059478/48‑1 (MQ=38)               cgcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                           >  1:1173628/1‑48 (MQ=38)               cgcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                           >  1:2098727/1‑48 (MQ=38)                gCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                         <  1:777798‑M1/49‑1 (MQ=255)                gCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                         <  1:27698‑M1/49‑1 (MQ=255)                  gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                         >  1:958418‑M1/1‑47 (MQ=255)                  gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                         >  1:2088843‑M1/1‑47 (MQ=255)                   atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                      >  1:1583261‑M1/1‑46 (MQ=255)                        tttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                  >  1:461464‑M1/1‑41 (MQ=255)                          tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                <  1:1596500‑M1/48‑10 (MQ=255)                            gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac             >  1:251168‑M1/1‑37 (MQ=255)                             cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc            >  1:173186‑M1/1‑36 (MQ=255)                                 gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag        <  1:45311‑M1/49‑18 (MQ=255)                                  cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc       >  1:548257‑M1/1‑31 (MQ=255)                                     tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc      <  1:433404‑M1/47‑20 (MQ=255)                                       aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacat  <  1:58730‑M1/49‑24 (MQ=255)                                       aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacat  <  1:146339‑M1/49‑24 (MQ=255)                                               |                                          TTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAA  >  NC_000913/765143‑765230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑