Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,866	Δ1 bp	coding (536/780 nt)	elyC ←	envelope biogenesis factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,863	0	G	.	100.0%	93.9 / NA	23	coding (539/780 nt)	elyC	envelope biogenesis factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/10);  total (13/10)

AAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTT  >  NC_000913/972815‑972905                                                 |                                           aaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAg                                            >  1:890953/1‑49 (MQ=255)  aGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgg                                           <  1:980434/49‑1 (MQ=255)     tcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgggcg                                        >  1:1282262/1‑47 (MQ=255)     tcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgggcg                                        >  1:40698/1‑47 (MQ=255)          gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCAc                                   >  1:2062299/1‑48 (MQ=39)            ccGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCaa                                >  1:701392/1‑49 (MQ=39)             cgcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAAt                               >  1:921727/1‑49 (MQ=39)              gcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATc                              <  1:1172533/49‑1 (MQ=39)                ggCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGc                            >  1:610970/1‑49 (MQ=39)                ggCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGc                            >  1:45734/1‑49 (MQ=39)                   aGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTg                         <  1:1485669/49‑1 (MQ=39)                   aGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTg                         <  1:1667135/49‑1 (MQ=39)                   aGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTg                         >  1:616231/1‑49 (MQ=39)                      tgtgATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGttt                      >  1:424173/1‑49 (MQ=39)                        tgATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTc                     <  1:1119594/48‑1 (MQ=39)                        tgATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCa                    >  1:2081385/1‑49 (MQ=39)                          aTGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCAct                  <  1:1515116/49‑1 (MQ=39)                             cGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActgct               <  1:190244/49‑1 (MQ=39)                             cGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActgc                <  1:716017/48‑1 (MQ=39)                                aGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCa            <  1:1090454/49‑1 (MQ=39)                                 ggTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAg           >  1:1569124/1‑49 (MQ=39)                                     aCCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttc       >  1:777810/1‑49 (MQ=39)                                     aCCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGctt        <  1:300456/48‑1 (MQ=39)                                      ccAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttct      >  1:787848/1‑49 (MQ=39)                                      ccAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttct      <  1:1438229/49‑1 (MQ=39)                                        agcagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttcttc    >  1:561291/1‑49 (MQ=39)                                          cagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttcttctt  >  1:2021584/1‑49 (MQ=39)                                                 |                                           AAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTT  >  NC_000913/972815‑972905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑