Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,400,314	Δ1 bp	coding (1460/1527 nt)	abgT ←	p‑aminobenzoyl glutamate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,400,314	0	A	.	100.0%	109.4 / NA	24	coding (1460/1527 nt)	abgT	p‑aminobenzoyl glutamate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (11/13);  total (11/13)

GGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGG  >  NC_000913/1400272‑1400348                                           |                                   ggACCTATCGGCAGACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCa                             >  1:858219/1‑48 (MQ=37) ggACCTATCGGCAGACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCa                             >  1:568878/1‑48 (MQ=37) ggACCTATCGGCAGACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCAt                            <  1:2065844/49‑1 (MQ=38)   aCCTATCGGCAGACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATac                          >  1:967859/1‑49 (MQ=38)     cTATCGGCAGACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACta                        >  1:1034266‑M1/1‑47 (MQ=255)         cGGCAGACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatg                      >  1:2032410‑M1/1‑43 (MQ=255)            cAGACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaa                 <  1:140347‑M1/49‑10 (MQ=255)            cAGACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcaga                  >  1:1600555‑M1/1‑40 (MQ=255)             aGACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaa                <  1:2055517‑M1/49‑11 (MQ=255)              gACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaac               <  1:1112054‑M1/49‑12 (MQ=255)              gACCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaac               <  1:825532‑M1/49‑12 (MQ=255)               aCCGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaac               >  1:930937‑M1/1‑37 (MQ=255)                ccGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaa             <  1:410493‑M1/49‑14 (MQ=255)                 cGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaat            <  1:751789‑M1/49‑15 (MQ=255)                 cGACAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaat            >  1:1670933‑M1/1‑35 (MQ=255)                   aCAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaatgc          >  1:1292828‑M1/1‑33 (MQ=255)                    cAAGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaatgct         <  1:354951‑M1/49‑18 (MQ=255)                      aGATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaatgctgt       <  1:764562‑M1/49‑20 (MQ=255)                       gATACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagagacaatgctgtc      <  1:894450‑M1/49‑21 (MQ=255)                        aTACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaatgctgtcg     <  1:2088056‑M1/49‑22 (MQ=255)                         tACCACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaatgctgtcga    >  1:1874669‑M1/1‑27 (MQ=255)                           ccACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaatgctgtcgatg  >  1:1947871‑M1/1‑25 (MQ=255)                           ccACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaatgctgtcgatg  <  1:315428‑M1/49‑25 (MQ=255)                           ccACGCCAGC‑ACATC‑GCAGCCATACtatgcagaaacaatgctgtcgat   <  1:1591789‑M1/48‑24 (MQ=255)                                           |                                   GGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGG  >  NC_000913/1400272‑1400348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑