Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,957	(G)6→7	coding (71/1386 nt)	yifK →	putative transporter YifK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,951	1	.	G	100.0%	53.4 / NA	16	L22R (CTG→CGG)	yifK	putative transporter YifK
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (11/5);  total (11/5)

AGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCG  >  NC_000913/3980906‑3980989                                               |                                       agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑ggg                                     >  1:660533/1‑49 (MQ=255)          gTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCa                             <  1:826905/49‑1 (MQ=39)             ggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTg                          >  1:1542273/1‑49 (MQ=39)              gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTgg                         <  1:645254/49‑1 (MQ=39)               cTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGc                        <  1:1954838/49‑1 (MQ=255)                tGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCg                       >  1:257410/1‑49 (MQ=255)                     gCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                  >  1:1135796/1‑49 (MQ=39)                     gCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                  >  1:817876/1‑49 (MQ=39)                     gCTCGACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGc                  >  1:1541262/1‑49 (MQ=39)                         gACATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTg               <  1:1029356/48‑1 (MQ=39)                           cATATCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGtt             >  1:1319204/1‑48 (MQ=39)                            atatCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTa           >  1:956815/1‑49 (MQ=39)                             tatCGAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTAt          <  1:525100/49‑1 (MQ=39)                                 gAACTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg      >  1:195374/1‑49 (MQ=39)                                  aaCTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATggggg     >  1:1668169/1‑49 (MQ=39)                                  aaCTCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATggggg     >  1:688959/1‑49 (MQ=39)                                     tCATCGCCCTGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccg  >  1:355514/1‑49 (MQ=39)                                               |                                       AGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCG  >  NC_000913/3980906‑3980989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑