Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,086	C→T	intergenic (‑300/‑77)	yaaI ← / → dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,086	0	C	T	100.0%	117.4 / NA	31	intergenic (‑300/‑77)	yaaI/dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/17);  total (14/17)

AGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCA  >  NC_000913/12041‑12129                                              |                                            agtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGt                                          >  1:247003/1‑49 (MQ=255) agtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGt                                          >  1:656763/1‑49 (MQ=255)  gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGt                                          <  1:1311016/48‑1 (MQ=255)     gtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGaa                                      <  1:1195816/49‑1 (MQ=255)      tCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGaaa                                     >  1:370130/1‑49 (MQ=255)          ccGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAg                                 <  1:1660595/49‑1 (MQ=255)          ccGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAg                                 <  1:71394/49‑1 (MQ=255)           cGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGa                                >  1:175679/1‑49 (MQ=255)           cGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGa                                <  1:1551543/49‑1 (MQ=255)             cAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACg                              <  1:1191458/49‑1 (MQ=255)             cAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACg                              >  1:848371/1‑49 (MQ=255)             cAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACg                              <  1:1200334/49‑1 (MQ=255)               gtgagtgaGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGtt                            <  1:844723/49‑1 (MQ=255)                  agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                          <  1:1537618/48‑1 (MQ=255)                  agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCg                         >  1:1534812/1‑49 (MQ=255)                   gtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGc                        >  1:538528/1‑49 (MQ=255)                      agtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGccc                      <  1:1208736/48‑1 (MQ=255)                      agtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGccc                      <  1:33130/48‑1 (MQ=255)                         cTGCAAAAAAATGAAATTGGGTAGTTGGAACCAGACGTTTCGCCCCTAt                  >  1:516046/1‑49 (MQ=255)                           gCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                >  1:169398/1‑49 (MQ=255)                            cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTAtt                 <  1:284109/47‑1 (MQ=255)                            cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                <  1:1501514/48‑1 (MQ=255)                             aaaaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                >  1:712331/1‑47 (MQ=255)                               aaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGa            <  1:169612/49‑1 (MQ=255)                               aaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGa            <  1:199090/49‑1 (MQ=255)                               aaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGa            <  1:1346268/49‑1 (MQ=255)                               aaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGa            >  1:1207518/1‑49 (MQ=255)                                      aaaTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACaa     >  1:1130656/1‑49 (MQ=255)                                        aTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAc    >  1:1647597/1‑48 (MQ=255)                                         ttGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACca  <  1:935155/49‑1 (MQ=255)                                          tGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACca  >  1:336099/1‑48 (MQ=255)                                              |                                            AGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCA  >  NC_000913/12041‑12129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑