Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,696	C→T	G103D (GGC→GAC)	asnC ←	DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,696	0	C	T	100.0%	134.6 / NA	34	G103D (GGC→GAC)	asnC	DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/14);  total (20/14)

CGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCT  >  NC_000913/3926652‑3926741                                             |                                              cGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGt                                           <  1:308724/49‑1 (MQ=255) cGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGt                                           <  1:1985638/49‑1 (MQ=255)   tcgatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtg                                         >  1:1344100/1‑49 (MQ=255)     gatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtgtg                                       <  1:1103268/49‑1 (MQ=255)     gatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtgtg                                       >  1:299815/1‑49 (MQ=255)     gatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtgtg                                       <  1:1251613/49‑1 (MQ=255)     gatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtgt                                        <  1:1149644/48‑1 (MQ=255)     gatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtgt                                        <  1:1685946/48‑1 (MQ=255)       tcgaACGGCAAATCACTTTTATAAAGATGCTGTAGTGGTCGGTTgtgt                                      >  1:992163/1‑48 (MQ=255)        cgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTaa                                    >  1:1944737/1‑49 (MQ=255)         gaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTaa                                    >  1:658847/1‑48 (MQ=255)            cGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAgg                                >  1:1782201/1‑49 (MQ=255)             ggCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGc                               >  1:421079/1‑49 (MQ=255)             ggCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGc                               >  1:347756/1‑49 (MQ=255)             ggCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGc                               >  1:2033370/1‑49 (MQ=255)                acaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                             >  1:422410/1‑48 (MQ=255)                 caTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCa                           <  1:1830379/49‑1 (MQ=255)                       ttttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACt                     >  1:1031018/1‑49 (MQ=255)                         ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACtt                    >  1:1450156/1‑48 (MQ=255)                         ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACt                     <  1:671462/47‑1 (MQ=255)                            taAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                <  1:1725454/49‑1 (MQ=255)                             aaaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                >  1:1155944/1‑48 (MQ=255)                             aaaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                >  1:1957124/1‑48 (MQ=255)                              aaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                >  1:356044/1‑47 (MQ=255)                               aGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAg             >  1:869204/1‑49 (MQ=255)                               aGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAg             <  1:1493484/49‑1 (MQ=255)                                  tGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCt          <  1:933525/49‑1 (MQ=255)                                  tGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCt          <  1:493642/49‑1 (MQ=255)                                     tGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTc       >  1:1036864/1‑49 (MQ=255)                                     tGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTc       >  1:1141928/1‑49 (MQ=255)                                      gTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTc       >  1:108111/1‑48 (MQ=255)                                       tAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCa     <  1:854274/49‑1 (MQ=255)                                         gTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGc   >  1:1900603/1‑49 (MQ=255)                                          tGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCt  <  1:1180361/49‑1 (MQ=255)                                             |                                              CGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCT  >  NC_000913/3926652‑3926741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑