Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,769	C→T	A390V (GCG→GTG)	wzyE →	enterobacterial common antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,769	0	C	T	100.0%	124.4 / NA	32	A390V (GCG→GTG)	wzyE	enterobacterial common antigen polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/15);  total (17/15)

TAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGT  >  NC_000913/3979725‑3979813                                             |                                             tAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATc                                          >  1:543497/1‑49 (MQ=255)      gCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCa                                      <  1:337799/48‑1 (MQ=255)      gCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCa                                      <  1:1773234/48‑1 (MQ=255)       cTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCaa                                     >  1:678898/1‑48 (MQ=255)       cTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCaa                                     >  1:324615/1‑48 (MQ=255)       cTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCaa                                     >  1:2001293/1‑48 (MQ=255)          taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATg                                 <  1:357121/49‑1 (MQ=255)          taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATg                                 <  1:1864334/49‑1 (MQ=255)          taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATg                                 <  1:2039645/49‑1 (MQ=255)            aaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGa                                <  1:757061/48‑1 (MQ=255)             aGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATc                              >  1:1326612/1‑49 (MQ=255)             aGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATc                              >  1:263254/1‑49 (MQ=255)              gCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCg                             <  1:1267862/49‑1 (MQ=255)               cTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                            >  1:258964/1‑49 (MQ=255)                  cGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGc                          <  1:733281/48‑1 (MQ=255)                    atatTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTgg                       >  1:944182/1‑49 (MQ=255)                    atatTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTgg                       >  1:1809358/1‑49 (MQ=255)                    atatTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTgg                       <  1:1435111/49‑1 (MQ=255)                       ttGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGgcgc                    <  1:378158/49‑1 (MQ=255)                         gCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGt                  >  1:51117/1‑49 (MQ=255)                           acaGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTg                 >  1:1288906/1‑48 (MQ=255)                           acaGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTg                 >  1:1131400/1‑48 (MQ=255)                              gTTACTGCTTTTGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAgg             >  1:1552988/1‑49 (MQ=255)                               tttCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAggg            >  1:112274/1‑49 (MQ=255)                                 tCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGc           <  1:1873363/48‑1 (MQ=255)                                 tCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCt          >  1:1894800/1‑49 (MQ=255)                                 tCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCt          >  1:1000311/1‑49 (MQ=255)                                    gCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGa       >  1:1475956/1‑49 (MQ=255)                                     cTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGAt      <  1:899048/49‑1 (MQ=255)                                        tGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTc    <  1:605300/48‑1 (MQ=255)                                         ggggTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCg   <  1:911081/48‑1 (MQ=255)                                          gggTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGt  <  1:506244/48‑1 (MQ=255)                                             |                                             TAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGT  >  NC_000913/3979725‑3979813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑