Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,983,666	T→C	F237L (TTC→CTC)	aslB →	putative anaerobic sulfatase maturation enzyme AslB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,983,666	0	T	C	100.0%	91.8 / NA	25	F237L (TTC→CTC)	aslB	putative anaerobic sulfatase maturation enzyme AslB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/11);  total (14/11)

TATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCG  >  NC_000913/3983623‑3983711                                            |                                              tatTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGt                                          >  1:991560/1‑49 (MQ=255)     cAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGt                                      >  1:159127/1‑49 (MQ=255)     cAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGt                                      >  1:1735243/1‑49 (MQ=255)      aGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGtt                                     <  1:989342/49‑1 (MQ=255)        ggTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTc                                   <  1:508005/49‑1 (MQ=255)        ggTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTc                                   >  1:382748/1‑49 (MQ=255)         gTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCt                                  >  1:854216/1‑49 (MQ=255)          tGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCtg                                 <  1:898570/49‑1 (MQ=255)          tGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCtg                                 <  1:460138/49‑1 (MQ=255)            aGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCtgg                                <  1:1437368/48‑1 (MQ=255)             gCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCtggtg                              <  1:1269141/49‑1 (MQ=255)               tAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGc                            >  1:1491773/1‑49 (MQ=255)                aaCGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGc                            >  1:1128713/1‑48 (MQ=255)                aaCGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGc                            >  1:778045/1‑48 (MQ=255)                    gAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGatat                       >  1:1570940/1‑49 (MQ=255)                           cGGTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTCGACCa                >  1:1083719/1‑49 (MQ=255)                               gcgcCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTg             >  1:528120/1‑48 (MQ=255)                               gcgcCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTg             >  1:1473749/1‑48 (MQ=255)                                cgcCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTgg            <  1:351071/48‑1 (MQ=255)                                cgcCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGa           >  1:564843/1‑49 (MQ=255)                                   cccGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATc         >  1:1892547/1‑48 (MQ=255)                                   cccGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCa        <  1:292090/49‑1 (MQ=255)                                      gaggagCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAAc     <  1:664100/49‑1 (MQ=255)                                        ggagCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAAcgc   <  1:2019595/49‑1 (MQ=255)                                         gagCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAAcgcg  <  1:1400083/49‑1 (MQ=255)                                            |                                              TATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCAAACGCG  >  NC_000913/3983623‑3983711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑