Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,093,223	A→G	V72V (GTT→GTC)	rhaM ←	L‑rhamnose mutarotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,093,223	0	A	G	100.0%	101.7 / NA	27	V72V (GTT→GTC)	rhaM	L‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/16);  total (11/16)

TTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATC  >  NC_000913/4093179‑4093268                                             |                                              ttAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt                                           >  1:1948601/1‑49 (MQ=255)   aGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATc                                          <  1:1637441/48‑1 (MQ=255)    gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCa                                         <  1:1625608/48‑1 (MQ=255)    gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCa                                         <  1:1634541/48‑1 (MQ=255)      gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                      <  1:2049956/49‑1 (MQ=255)          aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                  <  1:255733/49‑1 (MQ=255)             aCATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCaa                               >  1:855902/1‑49 (MQ=255)              cATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAAc                              <  1:792001/49‑1 (MQ=255)                tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                            <  1:1119364/49‑1 (MQ=255)                      atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTcc                      <  1:1170852/49‑1 (MQ=255)                      atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTcc                      <  1:780264/49‑1 (MQ=255)                      atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                       <  1:2053006/48‑1 (MQ=255)                      atatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                       >  1:339119/1‑48 (MQ=255)                       tatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCa                     <  1:168785/49‑1 (MQ=255)                             tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc               >  1:1912357/1‑49 (MQ=255)                             tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc               >  1:791464/1‑49 (MQ=255)                             tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc               >  1:984092/1‑49 (MQ=255)                                  caACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt             >  1:196387/1‑46 (MQ=255)                                  caACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt             >  1:1530613/1‑46 (MQ=255)                                  caACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAg          <  1:391931/49‑1 (MQ=255)                                     cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt       <  1:302306/49‑1 (MQ=255)                                      gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt       >  1:1642100/1‑48 (MQ=255)                                      gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt       >  1:1421395/1‑48 (MQ=255)                                      gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc      <  1:1731403/49‑1 (MQ=255)                                       ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc      >  1:139178/1‑48 (MQ=255)                                          gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtc  <  1:314006/49‑1 (MQ=255)                                          gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt   <  1:1189297/48‑1 (MQ=255)                                             |                                              TTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATC  >  NC_000913/4093179‑4093268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑