Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	149.8 / NA	38	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/20);  total (18/20)

CAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT  >  NC_000913/4438078‑4438168                                               |                                             cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                            >  1:1348961/1‑49 (MQ=255)  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                            <  1:1537438/48‑1 (MQ=255)   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                          <  1:117444/49‑1 (MQ=255)      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                       <  1:1161984/49‑1 (MQ=255)      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                       >  1:763864/1‑49 (MQ=255)       ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                      <  1:2075135/49‑1 (MQ=255)       ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                      <  1:292526/49‑1 (MQ=255)       ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                      >  1:1461288/1‑49 (MQ=255)         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                     >  1:497081/1‑48 (MQ=255)         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                    <  1:227935/49‑1 (MQ=255)            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                   >  1:693256/1‑47 (MQ=255)            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                   >  1:594547/1‑47 (MQ=255)            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc                                  <  1:233038/48‑1 (MQ=255)               ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                               >  1:1270997/1‑48 (MQ=255)                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                           <  1:706206/49‑1 (MQ=255)                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaa                            >  1:1527664/1‑48 (MQ=255)                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                          >  1:1571797/1‑48 (MQ=255)                      aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                       >  1:1263052/1‑49 (MQ=255)                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                     >  1:1554960/1‑49 (MQ=255)                          cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg                   <  1:1543072/49‑1 (MQ=255)                          cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTg                   <  1:460152/49‑1 (MQ=255)                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                  >  1:986209/1‑49 (MQ=255)                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                  >  1:595872/1‑48 (MQ=255)                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                <  1:1930177/49‑1 (MQ=255)                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt               <  1:2085278/49‑1 (MQ=255)                               cAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa              >  1:1231001/1‑49 (MQ=255)                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc             >  1:21646/1‑49 (MQ=255)                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAc             <  1:1200017/49‑1 (MQ=255)                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc            <  1:1185218/49‑1 (MQ=255)                                  aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc            >  1:1448285/1‑48 (MQ=255)                                  aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg           <  1:1138884/49‑1 (MQ=255)                                  aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg           <  1:60825/49‑1 (MQ=255)                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGa          >  1:397880/1‑48 (MQ=255)                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg      >  1:281378/1‑48 (MQ=255)                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg      <  1:683293/48‑1 (MQ=255)                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg      <  1:151059/48‑1 (MQ=255)                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAtt  <  1:1582695/49‑1 (MQ=255)                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt   <  1:927453/48‑1 (MQ=255)                                               |                                             CAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATT  >  NC_000913/4438078‑4438168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑