Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	50.1 / NA	14	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/12);  total (2/12)

ACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAG  >  NC_000913/988457‑988544                                           |                                              aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGa                                         <  1:1547628/49‑1 (MQ=255)       cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                    <  1:1505149/48‑1 (MQ=255)        gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                     >  1:1245888/1‑46 (MQ=255)             cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaaga                              <  1:1569189/48‑1 (MQ=255)              aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                            >  1:1570772/1‑49 (MQ=255)              aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                            <  1:898694/49‑1 (MQ=255)               ccTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                           <  1:1640769/49‑1 (MQ=255)                    ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                       <  1:844229/48‑1 (MQ=255)                     gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                     <  1:1759338/49‑1 (MQ=255)                     gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                     <  1:317578/49‑1 (MQ=255)                          aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                <  1:1556846/49‑1 (MQ=255)                             tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag             <  1:1895639/49‑1 (MQ=255)                             tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag             <  1:2083802/49‑1 (MQ=255)                                        gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg  <  1:1893487/49‑1 (MQ=255)                                           |                                              ACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAG  >  NC_000913/988457‑988544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑