Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,362	C→T	A84V (GCC→GTC)	mltG →	endolytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,362	0	C	T	100.0%	97.5 / NA	27	A84V (GCC→GTC)	mltG	endolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/8);  total (19/8)

AATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCT  >  NC_000913/1154320‑1154407                                           |                                              aaTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGAc                                         >  1:725319/1‑49 (MQ=255)  aTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACt                                        <  1:762038/49‑1 (MQ=255)   tGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACt                                        >  1:515728/1‑48 (MQ=255)     gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAc                                     <  1:1458673/49‑1 (MQ=255)      ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                    <  1:1153895/49‑1 (MQ=255)       tgctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCg                                   >  1:1909886/1‑49 (MQ=255)        gctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGc                                  >  1:879937/1‑49 (MQ=255)          tgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                                >  1:1116026/1‑49 (MQ=255)                tCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACAcc                          <  1:779754/49‑1 (MQ=255)                 cGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                         >  1:2028067/1‑49 (MQ=255)                  gAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                         >  1:205641/1‑48 (MQ=255)                    aCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                         >  1:259210/1‑46 (MQ=255)                      cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                    <  1:689718/49‑1 (MQ=255)                       ggATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                    >  1:706371/1‑48 (MQ=255)                       ggATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                    >  1:236833/1‑48 (MQ=255)                       ggATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                    >  1:613179/1‑48 (MQ=255)                        gATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGa                  >  1:507945/1‑49 (MQ=255)                           cTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCg               >  1:518982/1‑49 (MQ=255)                            tttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt              >  1:1687491/1‑49 (MQ=255)                            tttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt              >  1:1573223/1‑49 (MQ=255)                             ttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTg             >  1:1515882/1‑49 (MQ=255)                              tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAc                 <  1:2067111/44‑1 (MQ=255)                              tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt              <  1:187286/47‑1 (MQ=255)                              tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt              <  1:780058/47‑1 (MQ=255)                                     ttAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGAt     >  1:2063487/1‑49 (MQ=255)                                       aaaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGc   >  1:1048272/1‑49 (MQ=255)                                        aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCt  >  1:2055380/1‑49 (MQ=255)                                           |                                              AATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCT  >  NC_000913/1154320‑1154407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑