Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,240,439	C→T	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM → cvrA ←	protein YmgM putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,240,439	0	C	T	100.0%	93.9 / NA	24	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM cvrA	protein YmgM putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/9);  total (15/9)

CTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTT  >  NC_000913/1240394‑1240480                                              |                                          ctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTAcc                                        >  1:1922558/1‑49 (MQ=255) ctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTAc                                         <  1:1293489/48‑1 (MQ=255)   ttctCGGTCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGa                                      >  1:1872482/1‑49 (MQ=38)    tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCg                                       <  1:1840286/47‑1 (MQ=255)    tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGa                                      >  1:876204/1‑48 (MQ=255)    tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGa                                      <  1:1794971/48‑1 (MQ=255)      tcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACaa                                   >  1:2106845/1‑49 (MQ=255)         gCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCg                                >  1:387558/1‑49 (MQ=255)          ccACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCgg                               >  1:1488846/1‑49 (MQ=255)          ccACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCgg                               >  1:2087435/1‑49 (MQ=255)          ccACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCgg                               >  1:1081392/1‑49 (MQ=255)           cACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGt                              <  1:92130/49‑1 (MQ=255)            aCCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTg                             >  1:342475/1‑49 (MQ=255)              cGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGcc                           >  1:315641/1‑49 (MQ=255)               gTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCcg                          <  1:556133/49‑1 (MQ=255)                  cAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCgcgc                       >  1:754400/1‑49 (MQ=255)                          cccGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaacaac               >  1:233379/1‑49 (MQ=255)                           ccGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAAct              <  1:958682/49‑1 (MQ=255)                           ccGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAAct              <  1:1394563/49‑1 (MQ=255)                             ggCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgc            >  1:1723003/1‑49 (MQ=255)                              gCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgct           <  1:1814610/49‑1 (MQ=255)                               cAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgctg          <  1:2017257/49‑1 (MQ=255)                                 aaCTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGa        >  1:692109/1‑49 (MQ=255)                                       aCCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAAttt  >  1:488406/1‑49 (MQ=255)                                              |                                          CTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTT  >  NC_000913/1240394‑1240480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑