Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,562,085	C→T	A133T (GCA→ACA)	ddpA ←	putative D,D‑dipeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,562,085	0	C	T	100.0%	57.2 / NA	16	A133T (GCA→ACA)	ddpA	putative D,D‑dipeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/10);  total (6/10)

ACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAA  >  NC_000913/1562039‑1562128                                               |                                            aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGttt                                           >  1:1822554/1‑49 (MQ=255)  ccGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                          <  1:1256237/49‑1 (MQ=255)  ccGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                          <  1:349662/49‑1 (MQ=255)   cGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTct                                         <  1:1939865/49‑1 (MQ=255)     tATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgc                                       <  1:414785/49‑1 (MQ=255)      aTGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgct                                      >  1:393867/1‑49 (MQ=255)       tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgctg                                     >  1:1218710/1‑49 (MQ=255)       tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgct                                      <  1:2085205/48‑1 (MQ=255)           cgtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGccc                                 >  1:2057245/1‑49 (MQ=255)             tcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                               <  1:1655788/49‑1 (MQ=255)                 gAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc                           <  1:56230/49‑1 (MQ=255)                        aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                     <  1:1911285/48‑1 (MQ=255)                         aTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                     >  1:795572/1‑47 (MQ=255)                          tCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag                  >  1:692802/1‑49 (MQ=255)                              tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGc              >  1:1596314/1‑49 (MQ=255)                                aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCg            <  1:1163977/49‑1 (MQ=255)                                          aaaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaa  <  1:1559051/49‑1 (MQ=255)                                               |                                            ACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAA  >  NC_000913/1562039‑1562128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑