Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	73.8 / NA	20	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/12);  total (8/12)

TAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTT  >  NC_000913/118983‑119065                                              |                                      tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                    <  1:615365/49‑1 (MQ=255)   gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                   <  1:734330/48‑1 (MQ=255)    tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                   >  1:84262/1‑47 (MQ=255)     ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                >  1:1323065/1‑49 (MQ=255)     ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                >  1:1650608/1‑49 (MQ=255)        gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                             <  1:493406/49‑1 (MQ=255)         tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATc                            <  1:1109067/49‑1 (MQ=255)         tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATc                            <  1:682983/49‑1 (MQ=255)           tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                          >  1:1719234/1‑49 (MQ=255)             ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                        <  1:1995593/49‑1 (MQ=255)              tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                       >  1:441679/1‑49 (MQ=255)              tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                       >  1:1637180/1‑49 (MQ=255)                     gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                <  1:271728/49‑1 (MQ=255)                     gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                <  1:431034/49‑1 (MQ=255)                      aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg               <  1:280647/49‑1 (MQ=255)                         aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg            <  1:1413786/49‑1 (MQ=255)                          aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc           <  1:426392/49‑1 (MQ=255)                             tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt        >  1:253119/1‑49 (MQ=255)                              gcatggcatgCGGGAGCCTCTCAGCATCAGGGGCGCGAACGCTGCAAt        >  1:931994/1‑48 (MQ=39)                               catggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa      <  1:1237147/49‑1 (MQ=255)                                 tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt    >  1:147613/1‑49 (MQ=255)                                   gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtttt  <  1:792847/49‑1 (MQ=255)                                   gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt   <  1:1768332/48‑1 (MQ=255)                                              |                                      TAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTT  >  NC_000913/118983‑119065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑