Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,662,872	C→T	R773W (CGG→TGG)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,662,872	0	C	T	100.0%	76.7 / NA	20	R773W (CGG→TGG)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/9);  total (11/9)

CGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCA  >  NC_000913/1662832‑1662912                                         |                                         cGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                  >  1:2041653/1‑49 (MQ=255) cGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                  >  1:955059/1‑49 (MQ=255) cGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                  >  1:348028/1‑49 (MQ=255)  gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                  <  1:827404/48‑1 (MQ=255)  gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATc                                 <  1:452810/49‑1 (MQ=255)    gCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAc                               >  1:1942170/1‑49 (MQ=255)    gCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAc                               <  1:1615815/49‑1 (MQ=255)       aGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggc                            >  1:1414468/1‑49 (MQ=255)         gTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggcgg                          >  1:1185614/1‑49 (MQ=255)            cGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAg                       >  1:195446/1‑49 (MQ=255)             gTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGt                      <  1:339953/49‑1 (MQ=255)             gTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGt                      <  1:457090/49‑1 (MQ=255)                  ttAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCaa                 >  1:1789701/1‑49 (MQ=255)                  ttAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCaa                 >  1:861898/1‑49 (MQ=255)                      aGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATAtt             <  1:631672/49‑1 (MQ=255)                        cGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCt           <  1:1935513/49‑1 (MQ=255)                              tGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACctc     >  1:1142316/1‑49 (MQ=255)                                 ttGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCa  >  1:705556/1‑49 (MQ=255)                                  tGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCa  <  1:1786016/48‑1 (MQ=255)                                  tGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCa  <  1:1670688/48‑1 (MQ=255)                                         |                                         CGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCA  >  NC_000913/1662832‑1662912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑