Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,697,216	T→C	intergenic (‑164/+57)	uidR ← / ← hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,697,216	0	T	C	100.0%	86.7 / NA	23	intergenic (‑164/+57)	uidR/hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/13);  total (10/13)

ACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGT  >  NC_000913/1697174‑1697261                                           |                                              aCCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgatg                                          <  1:870408/48‑1 (MQ=255)     aTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGttt                                     <  1:166345/49‑1 (MQ=255)        gCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTAt                                   <  1:108852/48‑1 (MQ=255)        gCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTAt                                   <  1:1297087/48‑1 (MQ=255)         ccGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATcc                                 >  1:1044559/1‑49 (MQ=255)         ccGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATcc                                 >  1:1630894/1‑49 (MQ=255)          cGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATccc                                >  1:1102618/1‑49 (MQ=255)           gTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                               >  1:2047171/1‑49 (MQ=255)             gATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCgg                              >  1:1239986/1‑48 (MQ=255)                 tGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaaa                          <  1:1030204/48‑1 (MQ=255)                   gCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaaca                        <  1:278214/48‑1 (MQ=255)                      gCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacac                    >  1:303857/1‑49 (MQ=255)                       ccAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacac                    <  1:972650/48‑1 (MQ=255)                       ccAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacg                   <  1:1270782/49‑1 (MQ=255)                        cAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgc                  <  1:988075/49‑1 (MQ=255)                        cAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacg                   >  1:488901/1‑48 (MQ=255)                                  gCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgtt         <  1:2072686/48‑1 (MQ=255)                                  gCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgtt         >  1:142841/1‑48 (MQ=255)                                    agagACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAc      >  1:1051093/1‑49 (MQ=255)                                      agaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCg    <  1:166116/49‑1 (MQ=255)                                       gaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCgg   >  1:712057/1‑49 (MQ=255)                                        aCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGt  <  1:300243/49‑1 (MQ=255)                                        aCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGt  <  1:187136/49‑1 (MQ=255)                                           |                                              ACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGT  >  NC_000913/1697174‑1697261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑