Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,741,062	T→C	C384C (TGT→TGC)	ydhC →	putative transporter YdhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,741,062	0	T	C	100.0%	93.6 / NA	25	C384C (TGT→TGC)	ydhC	putative transporter YdhC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/12);  total (13/12)

ATCAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCA  >  NC_000913/1741017‑1741106                                              |                                             aTCAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCga                                            <  1:1423633/48‑1 (MQ=255)     aCAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGaagaag                                       >  1:586971/1‑49 (MQ=255)      cAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGt                                      <  1:289615/49‑1 (MQ=255)       agtagtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGtt                                     <  1:865800/49‑1 (MQ=255)         tagtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTgg                                   <  1:1531020/49‑1 (MQ=255)         tagtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTgg                                   >  1:468822/1‑49 (MQ=255)           gtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGc                                  <  1:1568442/48‑1 (MQ=255)              cTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGc                               <  1:1374985/48‑1 (MQ=255)                ggTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGc                               >  1:1513018/1‑46 (MQ=255)                 gTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGcct                             <  1:847501/47‑2 (MQ=255)                 gTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCa                             <  1:2078727/47‑1 (MQ=255)                 gTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAg                            <  1:1875058/48‑1 (MQ=255)                 gTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAg                            >  1:2052594/1‑48 (MQ=255)                     cgcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCa                       >  1:948367/1‑49 (MQ=255)                       cTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATg                     >  1:413558/1‑49 (MQ=255)                       cTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATg                     >  1:1995284/1‑49 (MQ=255)                        tGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATgg                    >  1:581137/1‑49 (MQ=255)                        tGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATgg                    >  1:1721951/1‑49 (MQ=255)                        tGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATgg                    >  1:716622/1‑49 (MQ=255)                            ttACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAAt                >  1:1257847/1‑49 (MQ=255)                              aCATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATg               <  1:703883/48‑1 (MQ=255)                               cATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGc              <  1:2110245/48‑1 (MQ=255)                                 tgatgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCg            >  1:14064/1‑48 (MQ=255)                                         cGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTc   <  1:1231758/49‑1 (MQ=255)                                           ttGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCa  >  1:2011493/1‑48 (MQ=255)                                              |                                             ATCAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCA  >  NC_000913/1741017‑1741106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑