Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	91.0 / NA	24	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/12);  total (12/12)

GCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAC  >  NC_000913/1742712‑1742798                                            |                                            gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                         >  1:1413882/1‑48 (MQ=255)     aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                    >  1:1958898/1‑49 (MQ=255)     aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                    <  1:1413222/49‑1 (MQ=255)      gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                   <  1:1828040/49‑1 (MQ=255)        tttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                 >  1:896012/1‑49 (MQ=255)           ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                               >  1:1799977/1‑48 (MQ=255)            tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                              >  1:1899649/1‑48 (MQ=255)                cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                         >  1:145486/1‑49 (MQ=255)                cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaa                          <  1:204183/48‑1 (MQ=255)                cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaa                          <  1:1967848/48‑1 (MQ=255)                 ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                        <  1:836673/49‑1 (MQ=255)                 ccAAGAGTCATGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                        >  1:478959/1‑49 (MQ=255)                    agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                     >  1:1116551/1‑49 (MQ=255)                      agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                   <  1:113012/49‑1 (MQ=255)                        tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                 >  1:351988/1‑49 (MQ=255)                          gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                <  1:1481214/48‑1 (MQ=255)                              gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc           >  1:120810/1‑49 (MQ=255)                              gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc           >  1:1570589/1‑49 (MQ=255)                                 ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt         <  1:1945277/48‑1 (MQ=255)                                  cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc        <  1:1380/48‑1 (MQ=255)                                    gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc     <  1:1275906/49‑1 (MQ=255)                                    gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc     <  1:638157/49‑1 (MQ=255)                                     tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc     >  1:260054/1‑48 (MQ=255)                                       tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc  <  1:2055382/49‑1 (MQ=255)                                            |                                            GCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAC  >  NC_000913/1742712‑1742798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑