Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,533	G→A	G316D (GGC→GAC)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,533	0	G	A	100.0%	74.4 / NA	20	G316D (GGC→GAC)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/12);  total (8/12)

AAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTC  >  NC_000913/1776488‑1776574                                              |                                          aaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGAcc                                         <  1:492330/48‑1 (MQ=255) aaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCt                                        >  1:1335905/1‑49 (MQ=255)  aGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTg                                       <  1:1274216/49‑1 (MQ=255)    gcgcGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGt                                     >  1:475163/1‑49 (MQ=255)         gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                <  1:1647198/49‑1 (MQ=255)          tGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCg                               <  1:1505890/49‑1 (MQ=255)          tGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCg                               <  1:246012/49‑1 (MQ=255)             ggAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCgaga                            <  1:1825915/49‑1 (MQ=255)                 tGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAgaagaa                        <  1:792231/49‑1 (MQ=255)                  gTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGaagaag                       >  1:1245384/1‑49 (MQ=255)                   tCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGaagaag                       <  1:1430266/48‑1 (MQ=255)                    cGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGt                     >  1:1174578/1‑49 (MQ=255)                      gCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtg                   <  1:93830/49‑1 (MQ=255)                        gTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgtg                 <  1:930773/49‑1 (MQ=255)                           ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCt               >  1:489522/1‑48 (MQ=255)                             tATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgat            <  1:1111948/49‑1 (MQ=255)                                  cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt       <  1:57140/49‑1 (MQ=255)                                   tGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt       >  1:606193/1‑48 (MQ=255)                                     aCGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTAt    >  1:120534/1‑49 (MQ=255)                                       ggCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTc  >  1:788372/1‑49 (MQ=255)                                              |                                          AAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTC  >  NC_000913/1776488‑1776574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑