Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,898,152	G→A	A407A (GCG→GCA)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,898,152	0	G	A	100.0%	108.9 / NA	28	A407A (GCG→GCA)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/12);  total (16/12)

CATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTG  >  NC_000913/1898107‑1898194                                              |                                           cATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACgg                                         <  1:1233419/49‑1 (MQ=255)    tGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATg                                      >  1:1984572/1‑49 (MQ=255)     gAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATg                                      <  1:575938/48‑1 (MQ=255)      aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATgg                                     <  1:256299/48‑1 (MQ=255)      aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGc                                    <  1:129878/49‑1 (MQ=255)        cGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctc                                  >  1:962348/1‑49 (MQ=255)        cGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctc                                  >  1:507101/1‑49 (MQ=255)         gTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctct                                 >  1:1596324/1‑49 (MQ=255)         gTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctct                                 <  1:1418714/49‑1 (MQ=255)           aaTGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgc                               >  1:646624/1‑49 (MQ=255)            aTGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgcg                              >  1:399608/1‑49 (MQ=255)             tGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgcgc                             >  1:1565120/1‑49 (MQ=255)                cATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGcgccg                           >  1:640526/1‑48 (MQ=255)                  ttGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCAc                        >  1:218694/1‑49 (MQ=255)                   tGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCAc                        >  1:1589876/1‑48 (MQ=255)                     ccTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAg                     >  1:1448841/1‑49 (MQ=255)                        ctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTg                   >  1:1521090/1‑48 (MQ=255)                         tgtgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCa                 >  1:1524977/1‑49 (MQ=255)                           tgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCAcc               >  1:1047650/1‑49 (MQ=255)                             aaGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACcgc             <  1:784959/49‑1 (MQ=255)                                  gATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtc        >  1:1676956/1‑49 (MQ=255)                                   aTTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtct       <  1:1170262/49‑1 (MQ=255)                                    ttAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc      <  1:876125/49‑1 (MQ=255)                                    ttAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc      <  1:2040839/49‑1 (MQ=255)                                    ttAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc      <  1:874654/49‑1 (MQ=255)                                    ttAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc      >  1:808615/1‑49 (MQ=255)                                        cgccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTg  <  1:36562/49‑1 (MQ=255)                                        cgccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTg  <  1:1123151/49‑1 (MQ=255)                                              |                                           CATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTG  >  NC_000913/1898107‑1898194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑