Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,784	A→G	V267A (GTC→GCC)	znuA ←	Zn(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,784	0	A	G	100.0%	85.8 / NA	23	V267A (GTC→GCC)	znuA	Zn(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/11);  total (12/11)

AGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACG  >  NC_000913/1941742‑1941828                                           |                                             aGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGctct                                        >  1:1561646/1‑49 (MQ=255)    ggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCaa                                     <  1:1613173/49‑1 (MQ=255)    ggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCaa                                     >  1:904390/1‑49 (MQ=255)     gATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAAc                                    >  1:786053/1‑49 (MQ=255)     gATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAAc                                    >  1:239102/1‑49 (MQ=255)     gATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAAc                                    <  1:490831/49‑1 (MQ=255)      aTCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACg                                   <  1:904653/49‑1 (MQ=255)         cAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGAcc                                <  1:943844/49‑1 (MQ=255)             gTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTg                            >  1:1670693/1‑49 (MQ=255)              ttCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTgg                           >  1:626937/1‑49 (MQ=255)                 ccATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCt                        <  1:1640492/49‑1 (MQ=255)                 ccATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCt                        <  1:651923/49‑1 (MQ=255)                    tACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGaa                     >  1:52758/1‑49 (MQ=255)                            gATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt             >  1:1609900/1‑49 (MQ=255)                             aTGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTc            <  1:1203804/49‑1 (MQ=255)                              tGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt             >  1:1425632/1‑47 (MQ=255)                                tCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGc         <  1:331419/49‑1 (MQ=255)                                 cccTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGc         >  1:1015987/1‑48 (MQ=255)                                   cTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaa      <  1:1766435/49‑1 (MQ=255)                                   cTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaa      <  1:1646844/49‑1 (MQ=255)                                     cGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaaaa    >  1:205340/1‑49 (MQ=255)                                      gTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAAc   <  1:968652/49‑1 (MQ=255)                                       tGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACg  >  1:297511/1‑49 (MQ=255)                                           |                                             AGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACG  >  NC_000913/1941742‑1941828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑