Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,885	C→T	T18I (ACC→ATC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,885	0	C	T	100.0%	85.2 / NA	22	T18I (ACC→ATC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/13);  total (9/13)

TGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAAT  >  NC_000913/214840‑214925                                              |                                         tgATTTCCCGACTTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                        <  1:795272/48‑1 (MQ=255)   aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGcc                                      <  1:1449095/48‑1 (MQ=255)   aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCa                                     >  1:488193/1‑49 (MQ=255)          gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                              >  1:363283/1‑49 (MQ=255)              tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                          <  1:38476/49‑1 (MQ=255)                ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAggg                        <  1:1015993/49‑1 (MQ=255)                  gCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                       <  1:1516748/48‑1 (MQ=255)                    tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                    >  1:592942/1‑49 (MQ=255)                      ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                   <  1:678858/48‑1 (MQ=255)                          cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg              <  1:875099/49‑1 (MQ=255)                           gATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg              >  1:1302310/1‑48 (MQ=255)                            aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg              >  1:1349028/1‑47 (MQ=255)                            aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg              >  1:1724464/1‑47 (MQ=255)                               gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagc          <  1:1561722/48‑1 (MQ=255)                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg       <  1:632100/48‑1 (MQ=255)                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt      >  1:1095123/1‑49 (MQ=255)                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt      >  1:504879/1‑49 (MQ=255)                                   gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt     <  1:216308/49‑1 (MQ=255)                                   gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt     <  1:195936/49‑1 (MQ=255)                                    cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta    <  1:1939318/49‑1 (MQ=255)                                      tGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa   >  1:142635/1‑48 (MQ=255)                                       gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat  <  1:545475/48‑1 (MQ=255)                                              |                                         TGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAAT  >  NC_000913/214840‑214925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑