Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,406,141	A→G	R167R (CGT→CGC)	lrhA ←	DNA‑binding transcriptional dual regulator LrhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,406,141	0	A	G	100.0%	83.1 / NA	22	R167R (CGT→CGC)	lrhA	DNA‑binding transcriptional dual regulator LrhA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/11);  total (11/11)

TTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTG  >  NC_000913/2406095‑2406180                                               |                                        tttGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCg                                       >  1:1540385/1‑49 (MQ=255)  ttGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCg                                       <  1:645059/48‑1 (MQ=255)  ttGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCg                                       <  1:2010777/48‑1 (MQ=255)     cAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAgg                                   <  1:847403/49‑1 (MQ=255)      aaTATGTATGCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGt                                  <  1:1347852/49‑1 (MQ=39)          tGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCaa                              >  1:704050/1‑49 (MQ=255)           gTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAAt                             >  1:1135849/1‑49 (MQ=255)            tATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATg                            <  1:913164/49‑1 (MQ=255)            tATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATg                            >  1:635633/1‑49 (MQ=255)            tATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATg                            >  1:634726/1‑49 (MQ=255)            tATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATg                            <  1:1228458/49‑1 (MQ=255)                ccGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATGCCt                         <  1:338697/48‑1 (MQ=255)                 cGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATGCCtt                        >  1:1779545/1‑48 (MQ=255)                 cGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATGCCtt                        >  1:1027321/1‑48 (MQ=255)                 cGCTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATGCCTTa                       <  1:1505404/49‑1 (MQ=255)                   cTGCGCAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATGCCTTaaa                     >  1:504296/1‑49 (MQ=255)                     gcgcAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATGCCTTAAACg                   >  1:166227/1‑49 (MQ=255)                      cgcAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATGCCTTAAACg                   <  1:1100170/48‑1 (MQ=255)                       gcAGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATGCCTTAAACGCt                 <  1:398496/49‑1 (MQ=255)                         aGTACCAGTGTGTTGGCGAGGTGCGCAGGTTCAATGCCTTAAACGCTg                >  1:1153884/1‑48 (MQ=255)                                  gtgtTGGCGAGGTGCGCAGGTTCAATGCCTTAAACGCTGAAGGACGATg      >  1:1119024/1‑49 (MQ=255)                                       ggCGAGGTGCGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGggtg  <  1:311049/48‑1 (MQ=255)                                               |                                        TTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTG  >  NC_000913/2406095‑2406180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑