Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,004	T→C	E43G (GAA→GGA)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,004	0	T	C	100.0%	107.9 / NA	27	E43G (GAA→GGA)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/17);  total (10/17)

CGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATC  >  NC_000913/2661960‑2662044                                             |                                         cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAGCAGTTGTCCCAg                                      <  1:1746080/49‑1 (MQ=39) cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAg                                      <  1:2008634/49‑1 (MQ=255)   cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGa                                     >  1:764106/1‑48 (MQ=255)      gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATaa                                  <  1:1717752/48‑1 (MQ=255)      gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATa                                   <  1:2117218/47‑1 (MQ=255)        aaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaa                               <  1:202891/49‑1 (MQ=255)        aaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                >  1:440374/1‑48 (MQ=255)         aCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaaa                              <  1:832473/49‑1 (MQ=255)              aCCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGaa                         <  1:614496/49‑1 (MQ=255)                 aTTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt                      >  1:1861581/1‑49 (MQ=255)                    tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc                   >  1:1579473/1‑49 (MQ=255)                    tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTcc                    <  1:2058956/48‑1 (MQ=255)                      tACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt                  <  1:538834/48‑1 (MQ=255)                        cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGa                <  1:1203405/48‑1 (MQ=255)                        cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGa                <  1:1405131/48‑1 (MQ=255)                        cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGAc               <  1:1182845/49‑1 (MQ=255)                         gCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACg              <  1:1780330/49‑1 (MQ=255)                         gCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACg              >  1:596971/1‑49 (MQ=255)                          cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt             >  1:1458058/1‑49 (MQ=255)                           aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt            >  1:558701/1‑49 (MQ=255)                           aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt            <  1:954276/49‑1 (MQ=255)                            gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc           <  1:2106624/49‑1 (MQ=255)                            gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc           >  1:96652/1‑49 (MQ=255)                              cGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg          >  1:70563/1‑48 (MQ=255)                                ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa        >  1:592426/1‑48 (MQ=255)                                     aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc  <  1:1274079/49‑1 (MQ=255)                                     aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc  <  1:1228808/49‑1 (MQ=255)                                             |                                         CGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATC  >  NC_000913/2661960‑2662044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑