Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,793,867	C→A	A378D (GCT→GAT)	gabT →	4‑aminobutyrate aminotransferase GabT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,793,867	0	C	A	100.0%	82.7 / NA	21	A378D (GCT→GAT)	gabT	4‑aminobutyrate aminotransferase GabT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/9);  total (12/9)

GATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCG  >  NC_000913/2793824‑2793910                                            |                                            gATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATcgcg                                        >  1:594790/1‑49 (MQ=255) gATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATcgcg                                        >  1:1844385/1‑49 (MQ=255)  aTCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCgcgc                                       >  1:972939/1‑49 (MQ=255)       aacaaGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCcgcg                                  <  1:170166/49‑1 (MQ=255)          aaGCCGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATa                               <  1:1507812/49‑1 (MQ=255)             ccGGACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAg                            <  1:1626592/49‑1 (MQ=255)               ggACGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGc                          >  1:1610623/1‑49 (MQ=255)                  cGCCAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTg                       <  1:2016910/49‑1 (MQ=255)                    ccAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGAt                     >  1:765855/1‑49 (MQ=255)                    ccAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGAt                     <  1:799688/49‑1 (MQ=255)                     cAAACTCACCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGAtt                    <  1:662409/49‑1 (MQ=255)                            aCCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATtcttct               <  1:773404/47‑1 (MQ=255)                            aCCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTtctct             >  1:405547/1‑49 (MQ=255)                            aCCGCCGAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTtctct             <  1:657039/49‑1 (MQ=255)                                ccgAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTg         >  1:655805/1‑49 (MQ=255)                                 cgAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGc        >  1:169171/1‑49 (MQ=255)                                 cgAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGc        >  1:909984/1‑49 (MQ=255)                                 cgAGATCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGc        >  1:1163519/1‑49 (MQ=255)                                   agaTCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCgg      <  1:2101080/49‑1 (MQ=255)                                    gaTCGTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGc     >  1:1148971/1‑49 (MQ=255)                                        gTGGATCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCg  >  1:1012808/1‑48 (MQ=255)                                            |                                            GATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCG  >  NC_000913/2793824‑2793910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑