Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	257,794	T→C	intergenic (+23/‑35)	frsA → / → crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	257,794	0	T	C	100.0%	133.9 / NA	33	intergenic (+23/‑35)	frsA/crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/14);  total (19/14)

GGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACC  >  NC_000913/257749‑257839                                              |                                              ggATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                            >  1:2028141/1‑49 (MQ=255)   aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                          <  1:2033171/49‑1 (MQ=255)    tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                         <  1:532592/49‑1 (MQ=255)    tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                          >  1:1226647/1‑48 (MQ=255)      gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       <  1:1896002/49‑1 (MQ=255)      gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       >  1:1660344/1‑49 (MQ=255)        aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       >  1:1916809/1‑47 (MQ=255)        aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                       >  1:386086/1‑47 (MQ=255)        aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACa                                     <  1:1373860/49‑1 (MQ=255)          aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                   >  1:2080074/1‑49 (MQ=255)          aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                   >  1:892180/1‑49 (MQ=255)           aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                  >  1:68404/1‑49 (MQ=255)             gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                 >  1:1538725/1‑48 (MQ=255)             gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                 >  1:1357635/1‑48 (MQ=255)               ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                              >  1:1808599/1‑49 (MQ=255)                 gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                             <  1:26208/48‑1 (MQ=255)                     tAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                         >  1:1391564/1‑48 (MQ=255)                      aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                         >  1:1542948/1‑47 (MQ=255)                       aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      <  1:1408830/49‑1 (MQ=255)                       aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                      >  1:55099/1‑49 (MQ=255)                          aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag                   <  1:1937451/49‑1 (MQ=255)                          aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag                   <  1:1634029/49‑1 (MQ=255)                           tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag                   <  1:226131/48‑1 (MQ=255)                            ttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                  >  1:822878/1‑48 (MQ=255)                             tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                  >  1:659576/1‑47 (MQ=255)                             tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                  >  1:495829/1‑47 (MQ=255)                              gCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg               >  1:1365689/1‑49 (MQ=255)                               cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc              <  1:1246853/49‑1 (MQ=255)                                  aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCa             <  1:1404317/47‑1 (MQ=255)                                    ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa         <  1:395094/49‑1 (MQ=255)                                      ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc        <  1:105178/48‑1 (MQ=255)                                          cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc   >  1:450398/1‑49 (MQ=255)                                           aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc  <  1:1786149/49‑1 (MQ=255)                                              |                                              GGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACC  >  NC_000913/257749‑257839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑