Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,364	T→C	V250A (GTC→GCC)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,364	0	T	C	100.0%	61.2 / NA	17	V250A (GTC→GCC)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/11);  total (6/11)

TCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTG  >  NC_000913/2813319‑2813401                                              |                                      tcatcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCt                                     <  1:1751808/48‑1 (MQ=255)   atcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTgg                                   <  1:1520325/48‑1 (MQ=255)     ccTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAg                                >  1:1402602/1‑49 (MQ=255)      cTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAg                                <  1:1376200/48‑1 (MQ=255)      cTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGc                               >  1:1420313/1‑49 (MQ=255)       ttACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCt                              >  1:1798891/1‑49 (MQ=255)          ccGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGa                            <  1:1195562/48‑1 (MQ=255)           cGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGAcc                          <  1:1017820/49‑1 (MQ=255)            gtggtgGCGGTGGGGGCTATCTGCTTCCTGATTGCCTGGGAGCTGAcc                          >  1:981948/1‑48 (MQ=38)                  gCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGAt                    <  1:153214/48‑1 (MQ=255)                  gCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGAt                    <  1:1279653/48‑1 (MQ=255)                  gCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGAt                    <  1:615865/48‑1 (MQ=255)                    ggtggtGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAc                 >  1:1370322/1‑49 (MQ=255)                     gtggtgGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAcc                <  1:1888905/49‑1 (MQ=255)                               tCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCg       <  1:122227/48‑1 (MQ=255)                                   cTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTg  >  1:1961863/1‑49 (MQ=255)                                   cTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTg  <  1:1108027/49‑1 (MQ=255)                                              |                                      TCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTG  >  NC_000913/2813319‑2813401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑