Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,052,352	C→A	W389C (TGG→TGT)	ubiH ←	2‑octaprenyl‑6‑methoxyphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,052,352	0	C	A	100.0%	67.1 / NA	18	W389C (TGG→TGT)	ubiH	2‑octaprenyl‑6‑methoxyphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/10);  total (8/10)

AACACTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACCCAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGTGAAT  >  NC_000913/3052306‑3052397                                               |                                              aaCACTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCcacaca                                             <  1:1409585/49‑1 (MQ=255)   cacTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAAc                                           <  1:734094/49‑1 (MQ=255)         gCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAgg                                      <  1:997213/48‑1 (MQ=255)          cATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGt                                    <  1:2031356/49‑1 (MQ=255)            ttGTTTATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGtgc                                  <  1:1995276/49‑1 (MQ=255)               tttATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGtgcgc                                <  1:1722535/48‑1 (MQ=255)                 tATTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGtgcgctg                              <  1:1833320/48‑1 (MQ=255)                  aTTCCTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGtgcgctgcg                            >  1:916344/1‑49 (MQ=255)                     ccTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGcc                          <  1:1357720/48‑1 (MQ=255)                     ccTTAAAACCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGcc                          <  1:481148/48‑1 (MQ=255)                         aaaaCCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGca                      >  1:1144685/1‑48 (MQ=255)                          aaaCCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGcaca                    >  1:1991996/1‑49 (MQ=255)                          aaaCCGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGcaca                    >  1:262262/1‑49 (MQ=255)                             ccGCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATcg                 >  1:1562116/1‑49 (MQ=255)                               gCCTTCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATcgcg               <  1:2013792/49‑1 (MQ=255)                                  ttCAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGc            >  1:746374/1‑49 (MQ=255)                                    cAACGCGCCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGcc           <  1:1843379/48‑1 (MQ=255)                                         cgcCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGt      >  1:1925557/1‑48 (MQ=255)                                          gcCACACAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGTg     >  1:1370561/1‑48 (MQ=255)                                            cacacaACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGTGaat  <  1:1762060/49‑1 (MQ=255)                                               |                                              AACACTTTGCATTGTTTATTCCTTAAAACCGCCTTCAACGCGCCACCCAACCGAGGGTGCGCTGCGCCAGCACATCGCGTGCCGGGGTGAAT  >  NC_000913/3052306‑3052397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑