| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,052,705 | G→A | H272Y (CAC→TAC) | ubiH ← | 2‑octaprenyl‑6‑methoxyphenol hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,052,705 | 0 | G | A | 95.8% | 85.8 / ‑3.3 | 24 | H272Y (CAC→TAC) | ubiH | 2‑octaprenyl‑6‑methoxyphenol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (9/14); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01 | |||||||||||
CATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGT > NC_000913/3052660‑3052747 | cATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTAGGt < 1:213538/49‑1 (MQ=255) ttGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTAGGTTa < 1:1066015/49‑1 (MQ=255) tGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTAGGTTaa > 1:266086/1‑49 (MQ=255) gCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTAGGTTAAc > 1:84281/1‑49 (MQ=255) cccACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTAGGTTAAc < 1:414631/48‑1 (MQ=255) cccACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTAGGTTAACg < 1:1133752/49‑1 (MQ=255) aCGGTACGATGGGTAATAGATCTGGCGGCGTAGGTTAACGCCAGCGGa > 1:996076/1‑48 (MQ=255) cGGTACGATGGGTAATAGATCTGGCGGCGTAGGTTAACGCCAGCGGAt > 1:1899588/1‑48 (MQ=255) cGGTACGATGGGTAATAGATCTGGCGGCGTAGGTTAACGCCAGCGGATa < 1:18531/49‑1 (MQ=255) aCGATGGGTAATAGATCTGGCGGCGTAGGTTAACGCCAGCGGATAAGCa > 1:1381219/1‑49 (MQ=255) cGATGGGTAATAGATCTGGCGGCGTAGGTTAACGCCAGCGGATAAGCAc < 1:1570174/49‑1 (MQ=255) gATGGGTAATAGATCTGGCGGCGTAGGTTAACGCCAGCGGATAAGCACt < 1:1622601/49‑1 (MQ=255) aTGGGTAATAGATCTGGCGGCGTAGGTTAACGCCAGCGGATAAGCACTg < 1:997285/49‑1 (MQ=255) gggTAATAGATCTGGCGGCGTAGGTTAACGCCAGCGGATAAGCACTGCg > 1:1511288/1‑49 (MQ=255) gTAATAGATCTGGCGGCGTAGGTTAACGCCAGCGGATAAGCACTGCGtt < 1:539187/49‑1 (MQ=255) tAATAGATCTGGCGGCGTAGGTTAACGCCAGCGGATAAGCACTGCGtt > 1:1641709/1‑48 (MQ=255) aTAGATCTGGCGGCGTAGGTTAACGCCAGCGGATAAGCACTGCGTTTa < 1:956921/48‑1 (MQ=255) aTAGATCTGGCGGCGTAGGTTAACGCCAGCGGATAAGCACTGCGTTTAc < 1:278020/49‑1 (MQ=255) tAGATCTGGCGGCGTAGGTTAACGCCAGCGGATAAGCACTGCGTTTAc > 1:932566/1‑48 (MQ=255) cTGGCGGCGTAGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGt > 1:540379/1‑49 (MQ=255) gcggcgTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTggg < 1:1830812/49‑1 (MQ=255) gcggcgTAGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTgg < 1:1003853/48‑1 (MQ=255) gcggcgTAGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTgg < 1:621943/48‑1 (MQ=255) cggcgTAGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGt < 1:1605401/49‑1 (MQ=255) | CATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGT > NC_000913/3052660‑3052747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |