Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,090,282	C→T	intergenic (+30/‑65)	yggI → / → endA	conserved protein YggI/DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,282	0	C	T	100.0%	133.4 / NA	34	intergenic (+30/‑65)	yggI/endA	conserved protein YggI/DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/25);  total (9/25)

AGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCT  >  NC_000913/3090236‑3090325                                               |                                            aGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTg                                           <  1:57799/49‑1 (MQ=255)  gCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTgg                                          <  1:256978/49‑1 (MQ=255)   cTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGc                                         >  1:737147/1‑49 (MQ=255)      gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCt                                        <  1:1947093/47‑1 (MQ=255)      gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGa                                      <  1:1460556/49‑1 (MQ=255)           gAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCa                                 >  1:1001137/1‑49 (MQ=255)           gAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCa                                 <  1:1722632/49‑1 (MQ=255)              aTAACCATCTGAACTATCAGGAACTTACCTGATTTGGCTGATTGCATAc                              <  1:1486322/49‑1 (MQ=39)               tAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAcc                             <  1:350044/49‑1 (MQ=255)                 aCCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCa                            >  1:538333/1‑48 (MQ=255)                    aTCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAAc                        <  1:2094065/49‑1 (MQ=255)                    aTCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAAAAAAAc                        <  1:278032/49‑1 (MQ=38)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACa                       <  1:286575/45‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCtt                   >  1:527913/1‑49 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCtt                   <  1:1981917/49‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCt                    <  1:181903/48‑1 (MQ=255)                          aCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCttt                  <  1:589368/49‑1 (MQ=255)                          aCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCttt                  >  1:2072331/1‑49 (MQ=255)                           cTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTc                 <  1:437151/49‑1 (MQ=255)                           cTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTc                 <  1:800431/49‑1 (MQ=255)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACATTTTTCGCt              <  1:1309922/49‑1 (MQ=38)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCg                <  1:1792649/47‑1 (MQ=255)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCg                <  1:454538/47‑1 (MQ=255)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCt              <  1:1342469/49‑1 (MQ=255)                               cAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTa             >  1:1328566/1‑49 (MQ=255)                                aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTAc            <  1:1385332/49‑1 (MQ=255)                                aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTAc            >  1:759267/1‑49 (MQ=255)                                 ggAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACg           <  1:2034210/49‑1 (MQ=255)                                 ggAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACg           <  1:165204/49‑1 (MQ=255)                                   aaCTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGtt         <  1:1525985/49‑1 (MQ=255)                                         ccTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGc   >  1:1117288/1‑49 (MQ=255)                                          cTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGc   <  1:352304/48‑1 (MQ=255)                                          cTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCt  >  1:1342937/1‑49 (MQ=255)                                          cTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCt  <  1:1000021/49‑1 (MQ=255)                                               |                                            AGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCT  >  NC_000913/3090236‑3090325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑