Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,091,305	A→G	S58G (AGC→GGC)	rsmE →	16S rRNA m(3)U1498 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,091,305	0	A	G	100.0%	101.3 / NA	27	S58G (AGC→GGC)	rsmE	16S rRNA m(3)U1498 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

TTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATC  >  NC_000913/3091260‑3091346                                              |                                          tttGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGc                                         <  1:1907372/48‑1 (MQ=255)   tGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaa                                       >  1:693228/1‑48 (MQ=255)      cGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaaaa                                   <  1:697517/49‑1 (MQ=255)      cGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaaaa                                   <  1:1315236/49‑1 (MQ=255)      cGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaaaa                                   <  1:471636/49‑1 (MQ=255)       ggTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCaaaaaaa                                  <  1:261578/49‑1 (MQ=255)              aCCAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTgg                            >  1:1709413/1‑48 (MQ=255)                cAGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAg                         >  1:262144/1‑49 (MQ=255)                 aGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGt                        <  1:717318/49‑1 (MQ=255)                 aGGTCTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGt                        >  1:1347792/1‑49 (MQ=255)                     cTTTGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAg                    >  1:856512/1‑49 (MQ=255)                      tttGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAgg                   >  1:318574/1‑49 (MQ=255)                        tGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                  <  1:1678987/48‑1 (MQ=255)                        tGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGt                  <  1:1075186/48‑1 (MQ=255)                        tGACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTg                 >  1:1940982/1‑49 (MQ=255)                         gACGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGc                >  1:193145/1‑49 (MQ=255)                           cGCCGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTg              <  1:1760510/49‑1 (MQ=255)                             ccGAAATTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTgg             >  1:366059/1‑48 (MQ=255)                                aaaTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAgg         <  1:2040657/49‑1 (MQ=255)                                 aaTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc        >  1:289014/1‑49 (MQ=255)                                 aaTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc        >  1:1302103/1‑49 (MQ=255)                                  aTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGcc       <  1:502513/49‑1 (MQ=255)                                  aTTACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGcc       <  1:690697/49‑1 (MQ=255)                                   ttACCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGc        >  1:1266343/1‑47 (MQ=255)                                    tACCAGCGCCGGCAAAAAAAGCGTGGAAGGGAAGGTGCTGGAAGGc        >  1:1919841/1‑46 (MQ=38)                                     aCCAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAg     <  1:663594/48‑1 (MQ=255)                                       cAGCGCCGGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATc  <  1:713931/49‑1 (MQ=255)                                              |                                          TTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATC  >  NC_000913/3091260‑3091346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑