Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	122.6 / NA	31	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/18);  total (13/18)

GAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477105‑3477195                                              |                                              gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                            >  1:510311/1‑49 (MQ=255)  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           <  1:5151/49‑1 (MQ=255)         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                    >  1:1577238/1‑49 (MQ=255)            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                 <  1:1785817/49‑1 (MQ=255)            gagTCTGTTCGATCTCGTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  <  1:698607/48‑1 (MQ=255)              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               >  1:1182863/1‑49 (MQ=255)                  gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            >  1:1268105/1‑48 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             <  1:688483/46‑1 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             <  1:376878/46‑1 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            <  1:973290/47‑1 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            <  1:951455/47‑1 (MQ=255)                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         >  1:333416/1‑48 (MQ=255)                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:1259955/1‑48 (MQ=255)                         ctctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:177298/1‑47 (MQ=255)                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   >  1:333/1‑49 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      <  1:69131/44‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                     <  1:2086669/45‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                     <  1:1440796/45‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:558417/47‑1 (MQ=255)                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:151943/46‑1 (MQ=255)                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:1165347/46‑1 (MQ=255)                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:1308278/46‑1 (MQ=255)                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                <  1:954255/48‑1 (MQ=255)                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               >  1:1408917/1‑49 (MQ=255)                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg            <  1:444743/48‑1 (MQ=255)                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           >  1:1071300/1‑49 (MQ=255)                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:1462305/1‑49 (MQ=255)                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       <  1:1306188/49‑1 (MQ=255)                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       >  1:1559072/1‑49 (MQ=255)                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:1657129/48‑1 (MQ=255)                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa  >  1:844347/1‑49 (MQ=255)                                              |                                              GAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477105‑3477195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑