Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,495,261	A→G	K417K (AAA→AAG)	nirB →	nitrite reductase catalytic subunit NirB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,495,261	0	A	G	100.0%	66.6 / NA	18	K417K (AAA→AAG)	nirB	nitrite reductase catalytic subunit NirB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

CCAGCACACTCGGGTAGCGGCAAGCCGTCTATCGGTGTTGATAAACTGCCGGACAGCGCGCAAATCTGCTCCTGCTTCGACGT  >  NC_000913/3495217‑3495299                                             |                                       ccAGCACACTCGGGTAGCGGCAAGCCGTCTATCGGTGTTGATAAGCt                                      <  1:122215/47‑1 (MQ=255) ccAGCACACTCGGGTAGCGGCAAGCCGTCTATCGGTGTTGATAAGCTGc                                    <  1:1982269/49‑1 (MQ=255)            gggTAGCGGCAAGCCGTCTATCGGTGTTGATAAGCTGCCGGACAgcgc                          <  1:1687897/48‑1 (MQ=255)             ggTAGCGGCAAGCCGTCTATCGGTGTTGATAAGCTGCCGGACAgcgcg                         >  1:1632377/1‑48 (MQ=255)                  cGGCAAGCCGTCTATCGGTGTTGATAAGCTGCCGGACAGCGCGCAAATc                   <  1:1404465/49‑1 (MQ=255)                    gCAAGCCGTCTATCGGTGTTGATAAGCTGCCGGACAGCGCGCAAATCTg                 <  1:676492/49‑1 (MQ=255)                    gCAAGCCGTCTATCGGTGTTGATAAGCTGCCGGACAACGCGCAAATCTg                 <  1:1961648/49‑1 (MQ=38)                        gCCGTCTATCGGTGTTGATAAGCTGCCGGACAGCGCGCAAATCTGCTcc             <  1:1488316/49‑1 (MQ=255)                         ccGTCTATCGGTGTTGATAAGCTGCCGGACAGCGCGCAAATCTGCTCCt            <  1:931658/49‑1 (MQ=255)                          cGTCTATCGGTGTTGATAAGCTGCCGGACAGCGCGCAAATCTGCTCCTg           >  1:1159810/1‑49 (MQ=255)                          cGTCTATCGGTGTTGATAAGCTGCCGGACAGCGCGCAAATCTGCTCCTg           >  1:512297/1‑49 (MQ=255)                          cGTCTATCGGTGTTGATAAGCTGCCGGACAGCGCGCAAATCTGCTCCTg           >  1:115771/1‑49 (MQ=255)                             cTATCGGTGTTGATAAGCTGCCGGACAGCGCGCAAATCTGCTCCTGCtt        >  1:875115/1‑49 (MQ=255)                               aTCGGTGTTGATAAGCTGCCGGACAGCGCGCAAATCTGCTCCTGCTTc       >  1:560858/1‑48 (MQ=255)                                tCGGTGTTGATAAGCTGCCGGACAGCGCGCAAATCTGCTCCTGCtt        <  1:2035270/46‑1 (MQ=255)                                  ggTGTTGATAAGCTGCCGGACAGCGCGCAAATCTGCTCCTGCTTCGAc    <  1:925184/48‑1 (MQ=255)                                   gtgtTGATAAGCTGCCGGACAGCGCGCAAATCTGCTCCTGCTTCGACGt  >  1:869510/1‑49 (MQ=255)                                   gtgtTGATAAGCTGCCGGACAGCGCGCAAATCTGCTCCTGCTTCGACGt  >  1:1091001/1‑49 (MQ=255)                                             |                                       CCAGCACACTCGGGTAGCGGCAAGCCGTCTATCGGTGTTGATAAACTGCCGGACAGCGCGCAAATCTGCTCCTGCTTCGACGT  >  NC_000913/3495217‑3495299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑