Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	131.1 / NA	34	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/15);  total (19/15)

GGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512958‑3513048                                              |                                              ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTc                                             <  1:968049/48‑1 (MQ=255)     tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:1792653/49‑1 (MQ=255)      cAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:991749/1‑49 (MQ=255)       aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:718000/49‑1 (MQ=255)       aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:88645/49‑1 (MQ=255)        gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:858882/1‑47 (MQ=255)        gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:272179/1‑47 (MQ=255)          gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  1:1804898/1‑49 (MQ=255)           aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:1545552/1‑49 (MQ=255)           aCCCCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:775027/1‑49 (MQ=39)            ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:1103418/1‑49 (MQ=255)             cGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:1840345/1‑48 (MQ=255)               cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              <  1:1450768/49‑1 (MQ=255)                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:1745885/49‑1 (MQ=255)                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:1743663/49‑1 (MQ=255)                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:1685491/49‑1 (MQ=255)                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:1648061/49‑1 (MQ=255)                 cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            <  1:847991/49‑1 (MQ=255)                  gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           >  1:1033423/1‑49 (MQ=255)                  gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           <  1:766854/49‑1 (MQ=255)                        ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  1:728494/1‑49 (MQ=255)                          ttcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    <  1:1955036/48‑1 (MQ=255)                             ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:508148/1‑49 (MQ=255)                             ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:109075/1‑49 (MQ=255)                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              >  1:401724/1‑49 (MQ=255)                                 cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            >  1:1975042/1‑49 (MQ=255)                                    ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:1875228/1‑48 (MQ=255)                                    ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          <  1:1302960/48‑1 (MQ=255)                                    ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTc         <  1:1839735/49‑1 (MQ=255)                                    ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTc         <  1:1470784/49‑1 (MQ=255)                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAACGt          >  1:1283450/1‑46 (MQ=39)                                       aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:542808/1‑49 (MQ=255)                                           cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:347527/1‑49 (MQ=255)                                           cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:954617/1‑49 (MQ=255)                                              |                                              GGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512958‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑